Role of two series of Ca2+ oscillations in activation of ascidian eggs

Manabu Yoshida, Noburu Sensui, Takafumi Inoue, Masaaki Morisawa, Katsuhiko Mikoshiba

研究成果: Article

49 引用 (Scopus)

抄録

Changes in [Ca2+](i) are an essential factor regulating egg activation. Matured ascidian eggs are arrested at metaphase I, and two series of [Ca2+](i) transients have been observed after fertilization: Ca2+ waves just after fertilization (Series I) and [Ca2+](i) oscillation between the first and second polar body extrusion (Series II). We investigated mechanisms involved in the elevation of [Ca2+](i) and the role of the [Ca2+](i) transients during egg activation in Ciona savignyi. The monoclonal antibody 18A10 against IP3 receptor type 1, which inhibits IP3- induced Ca2+ release in hamster and mouse eggs, did not show substantial inhibitory effects on series I or egg deformation, whereas Series II and the first cell division were inhibited by the antibody. Ruthenium red, an inhibitor of ryanodine receptor-mediated Ca2+ release, had no apparent effect of [Ca2+](i) transients and other events related to the egg activation. Microinjection of IP3 into unfertilized eggs induced [Ca2+](i) transients similar to those seen in Series I, whereas injection of cyclic ADP ribose, an agonist of ryanodine receptors, rarely induced [Ca2+](i) transient. Adenophostin B, a potent nonmetabolizable agonist of IP3 receptors, induced [Ca2+](i) oscillations which continued after first polar body extrusion, without separation to two series, and led to extrusion of first and second polar bodies. These results suggest that Series II is driven by the mouse type 1-like IP3 receptor while Series I seems to be mediated by another type of IP3 receptor. Injection of IP3 only induced the first polar body extrusion and the egg was arrested at metaphase II even when a higher amount of IP3 was injected. On the other hand, reinjection of IP3 after the first polar body extrusion led to emission of the second polar body. Thus, Series I and II of [Ca2+](i) transients are likely to be required for metaphase-anaphase transition in meiosis.

元の言語English
ページ(範囲)122-133
ページ数12
ジャーナルDevelopmental Biology
203
発行部数1
DOI
出版物ステータスPublished - 1998 11 1
外部発表Yes

Fingerprint

Polar Bodies
Urochordata
Eggs
Ovum
Inositol 1,4,5-Trisphosphate Receptors
Metaphase
Ryanodine Receptor Calcium Release Channel
Fertilization
Cyclic ADP-Ribose
Ruthenium Red
Anaphase
Injections
Meiosis
Microinjections
Cricetinae
Cell Division
Monoclonal Antibodies
Antibodies

ASJC Scopus subject areas

  • Developmental Biology

これを引用

Role of two series of Ca2+ oscillations in activation of ascidian eggs. / Yoshida, Manabu; Sensui, Noburu; Inoue, Takafumi; Morisawa, Masaaki; Mikoshiba, Katsuhiko.

:: Developmental Biology, 巻 203, 番号 1, 01.11.1998, p. 122-133.

研究成果: Article

Yoshida, M, Sensui, N, Inoue, T, Morisawa, M & Mikoshiba, K 1998, 'Role of two series of Ca2+ oscillations in activation of ascidian eggs', Developmental Biology, 巻. 203, 番号 1, pp. 122-133. https://doi.org/10.1006/dbio.1998.9037
Yoshida, Manabu ; Sensui, Noburu ; Inoue, Takafumi ; Morisawa, Masaaki ; Mikoshiba, Katsuhiko. / Role of two series of Ca2+ oscillations in activation of ascidian eggs. :: Developmental Biology. 1998 ; 巻 203, 番号 1. pp. 122-133.
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