Rolling properties of rGPIBα-conjugated phospholipid vesicles with different membrane flexibilities on vWf surface under flow conditions

Shinji Takeoka, Yuji Teramura, Yosuke Okamura, Eishun Tsuchida, Makoto Handa, Yasuo Ikeda

研究成果: Article査読

36 被引用数 (Scopus)

抄録

The recombinant fragment of the platelet membrane glycoprotein, rGPIb́, was conjugated to phospholipid vesicles with the average diameter of ca. 1 μm using N-succinimidyl 3-(2-pyridyldithio) propionate (SPDP). We used five kinds of rGPIb́-vesicles with different fluorescent anisotropies of 1,6-diphenyl-1,3,5-hexatriene (DPH) to study the rolling properties of the vesicles on the von Willebrand factor (vWf)-immobilized surface. Under flow conditions, the rolling velocity of the rGPIb́-vesicles decreased with the increasing membrane flexibility. It is considered that the vesicles with a high membrane flexibility have a high deformability and can be flattened to a high degree during rolling on the vWf surface, thus resulting in the large contact area. We obtained a recipe to control the rolling velocity of artificial platelets by membrane flexibility.

本文言語English
ページ(範囲)765-770
ページ数6
ジャーナルBiochemical and Biophysical Research Communications
296
3
DOI
出版ステータスPublished - 2002

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

フィンガープリント 「Rolling properties of rGPIBα-conjugated phospholipid vesicles with different membrane flexibilities on vWf surface under flow conditions」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル