Room temperature uniform and high throughput agarose gel micro droplet generation for single cell analysis

T. Hirose, Y. Hoshino, D. H. Yoon, T. Mori, T. Sekiguchi, H. Takeyama, S. Shoji

研究成果: Conference contribution

抄録

Uniform agarose gel micro droplet (GMD) generation method was developed using simple cross-junction microfluidic device without precise temperature control. Ultralow gelling temperature agarose was used as cell-enclosing capsule material because it remains in liquid phase from 20 °C to 45 °C. About 50 μm in diameter GMD which is suitable size for the target cell culturing media is generated with generation rate of about 200 drops/sec and size deviation smaller than 3.83 %. Encapsulated single E.coli cell was cultured successfully in the agarose GMD for 3 hours. Single cell encapsulated GMD generation ratio was about 31 %.

本文言語English
ホスト出版物のタイトル17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
出版社Chemical and Biological Microsystems Society
ページ440-442
ページ数3
ISBN(印刷版)9781632666246
出版ステータスPublished - 2013 1 1
イベント17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
継続期間: 2013 10 272013 10 31

出版物シリーズ

名前17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
1

Conference

Conference17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
CountryGermany
CityFreiburg
Period13/10/2713/10/31

ASJC Scopus subject areas

  • Bioengineering

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