Room temperature uniform and high throughput agarose gel micro droplet generation for single cell analysis

研究成果: Conference contribution

抄録

Uniform agarose gel micro droplet (GMD) generation method was developed using simple cross-junction microfluidic device without precise temperature control. Ultralow gelling temperature agarose was used as cell-enclosing capsule material because it remains in liquid phase from 20 °C to 45 °C. About 50 μm in diameter GMD which is suitable size for the target cell culturing media is generated with generation rate of about 200 drops/sec and size deviation smaller than 3.83 %. Encapsulated single E.coli cell was cultured successfully in the agarose GMD for 3 hours. Single cell encapsulated GMD generation ratio was about 31 %.

元の言語English
ホスト出版物のタイトル17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
出版者Chemical and Biological Microsystems Society
ページ440-442
ページ数3
1
ISBN(印刷物)9781632666246
出版物ステータスPublished - 2013
イベント17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg
継続期間: 2013 10 272013 10 31

Other

Other17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Freiburg
期間13/10/2713/10/31

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Sepharose
Gels
Throughput
Temperature
Microfluidics
Temperature control
Escherichia coli
Capsules
Liquids

ASJC Scopus subject areas

  • Bioengineering

これを引用

Hirose, T., Hoshino, Y., Yoon, D., Mori, T., Sekiguchi, T., Takeyama, H., & Shoji, S. (2013). Room temperature uniform and high throughput agarose gel micro droplet generation for single cell analysis. : 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (巻 1, pp. 440-442). Chemical and Biological Microsystems Society.

Room temperature uniform and high throughput agarose gel micro droplet generation for single cell analysis. / Hirose, T.; Hoshino, Y.; Yoon, Donghyun; Mori, T.; Sekiguchi, Tetsushi; Takeyama, Haruko; Shoji, Shuichi.

17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. 巻 1 Chemical and Biological Microsystems Society, 2013. p. 440-442.

研究成果: Conference contribution

Hirose, T, Hoshino, Y, Yoon, D, Mori, T, Sekiguchi, T, Takeyama, H & Shoji, S 2013, Room temperature uniform and high throughput agarose gel micro droplet generation for single cell analysis. : 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. 巻. 1, Chemical and Biological Microsystems Society, pp. 440-442, 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, Freiburg, 13/10/27.
Hirose T, Hoshino Y, Yoon D, Mori T, Sekiguchi T, Takeyama H その他. Room temperature uniform and high throughput agarose gel micro droplet generation for single cell analysis. : 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. 巻 1. Chemical and Biological Microsystems Society. 2013. p. 440-442
Hirose, T. ; Hoshino, Y. ; Yoon, Donghyun ; Mori, T. ; Sekiguchi, Tetsushi ; Takeyama, Haruko ; Shoji, Shuichi. / Room temperature uniform and high throughput agarose gel micro droplet generation for single cell analysis. 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. 巻 1 Chemical and Biological Microsystems Society, 2013. pp. 440-442
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abstract = "Uniform agarose gel micro droplet (GMD) generation method was developed using simple cross-junction microfluidic device without precise temperature control. Ultralow gelling temperature agarose was used as cell-enclosing capsule material because it remains in liquid phase from 20 °C to 45 °C. About 50 μm in diameter GMD which is suitable size for the target cell culturing media is generated with generation rate of about 200 drops/sec and size deviation smaller than 3.83 {\%}. Encapsulated single E.coli cell was cultured successfully in the agarose GMD for 3 hours. Single cell encapsulated GMD generation ratio was about 31 {\%}.",
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AU - Sekiguchi, Tetsushi

AU - Takeyama, Haruko

AU - Shoji, Shuichi

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KW - Gel micro droplet (GMD)

KW - Microfluidics

KW - Single cell

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