Rotation of F1-ATPase and the hinge residues of the β subunit

Tomoko Masaike, Noriyo Mitome, Hiroyuki Noji, Eiro Muneyuki, Ryohei Yasuda, Kazuhiko Kinosita, Masasuke Yoshida*

*この研究の対応する著者

研究成果査読

51 被引用数 (Scopus)

抄録

Rotation of a motor protein, F1-ATPase, was demonstrated using a unique single-molecule observation system. This paper reviews what has been clarified by this system and then focuses on the role of residues at the hinge region of the β subunit. We have visualised rotation of a single molecule of F1-ATPase by attaching a fluorescent actin filament to the top of the γ subunit in the immobilised F1-ATPase, thus settling a major controversy regarding the rotary catalysis. The rotation of the γ subunit was exclusively in one direction, as could be predicted by the crystal structure of bovine heart F1-ATPase. Rotation at low ATP concentrations revealed that one revolution consists of three 120°steps, each fuelled by the binding of an ATP to the β subunit. The mean work done by a 120°step was approximately 80 pN nm, a value close to the free energy liberated by hydrolysis of one ATP molecule, implying nearly 100 % efficiency of energy conversion. The torque is probably generated by the β subunit, which undergoes large opening-closing domain motion upon binding of AT(D)P. We identified three hinge residues, βHis179, βGly180 and βGly181, whose peptide bond dihedral angles are drastically changed during domain motion. Simultaneous substitution of these residues with alanine resulted in nearly complete loss (99 %) of ATPase activity. Single or double substitution of the two Gly residues did not abolish the ATPase activity. However, reflecting the shift of the equilibrium between the open and closed forms of the β subunit, single substitution caused changes in the propensity to generate the kinetically trapped Mg-ADP inhibited form: Gly180Ala enhanced the propensity and Gly181Ala abolished the propensity. In spite of these changes, the mean rotational torque was not changed significantly for any of the mutants.

本文言語English
ページ(範囲)1-8
ページ数8
ジャーナルJournal of Experimental Biology
203
1
出版ステータスPublished - 2000 1
外部発表はい

ASJC Scopus subject areas

  • 農業および生物科学(全般)
  • 農業および生物科学(その他)

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