Saccharomonospora colocasiae sp. Nov., an actinomycete isolated from the rhizosphere of Colocasia esculenta

Watsapon Wattanasuepsin, Bungonsiri Intra, Akira Take, Yuki Inahashi, Jirayut Euanorasetr, Satoshi Ōmura, Atsuko Matsumoto, Watanalai Panbangred

研究成果: Article

1 引用 (Scopus)

抄録

A non-Streptomyces actinomycete, designated as strain S265T, was isolated from rhizosphere collected under an elephant ear plant (Colocasia esculenta) in Bangkok, Thailand. The taxonomic position of this strain was determined by a polyphasic approach. Strain S265T formed single globose spores on long, branching, aerial hyphae. It produced abundant aerial mycelium with green colour. The cell wall contained meso-diaminopimelic acid, and diagnostic whole-cell sugars were arabinose and galactose. Phosphatidylethanolamine and diphosphatidylglycerol were detected predominantly as polar lipids, whereas mycolic acids were not found. The major menaquinone was MK-9(H4), and principal cellular fatty acids were C15: 1 B, iso-C16: 1 H, anteiso-C15: 0 and C15: 0 2-OH. The DNA G+C content was 69 mol%. According to phylogenetic analysis, strain S265T was clustered with Saccharomonospora glauca K62T (98.1%) and Saccharomonospora viridis DSM 43017T (97.1%) despite its 16S rRNA gene sequence showing the highest similarity value to that of Saccharomonospora azurea NA-128T (98.6%). DNA–DNA relatedness values between strain S265T and the closely related strains were in the range of 7–50%, thus strengthening the evidence derived from the polyphasic study that strain S265T represents a novel species within the genus Saccharomonospora, for which the name Saccharomonospora colocasiae sp. nov. is proposed. The type strain is S265T (=TBRC 7235T=NBRC 112945T).

元の言語English
ページ(範囲)4572-4577
ページ数6
ジャーナルInternational Journal of Systematic and Evolutionary Microbiology
67
発行部数11
DOI
出版物ステータスPublished - 2017 11 1
外部発表Yes

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Saccharomonospora
Colocasia
Mycolic Acids
Diaminopimelic Acid
Vitamin K 2
Colocasia esculenta
Rhizosphere
Arabinose
Cardiolipins
Hyphae
Actinobacteria
Mycelium
Base Composition
Thailand
Spores
Galactose
rRNA Genes
Cell Wall
Names
Ear

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

これを引用

Saccharomonospora colocasiae sp. Nov., an actinomycete isolated from the rhizosphere of Colocasia esculenta. / Wattanasuepsin, Watsapon; Intra, Bungonsiri; Take, Akira; Inahashi, Yuki; Euanorasetr, Jirayut; Ōmura, Satoshi; Matsumoto, Atsuko; Panbangred, Watanalai.

:: International Journal of Systematic and Evolutionary Microbiology, 巻 67, 番号 11, 01.11.2017, p. 4572-4577.

研究成果: Article

Wattanasuepsin, W, Intra, B, Take, A, Inahashi, Y, Euanorasetr, J, Ōmura, S, Matsumoto, A & Panbangred, W 2017, 'Saccharomonospora colocasiae sp. Nov., an actinomycete isolated from the rhizosphere of Colocasia esculenta', International Journal of Systematic and Evolutionary Microbiology, 巻. 67, 番号 11, pp. 4572-4577. https://doi.org/10.1099/ijsem.0.002336
Wattanasuepsin, Watsapon ; Intra, Bungonsiri ; Take, Akira ; Inahashi, Yuki ; Euanorasetr, Jirayut ; Ōmura, Satoshi ; Matsumoto, Atsuko ; Panbangred, Watanalai. / Saccharomonospora colocasiae sp. Nov., an actinomycete isolated from the rhizosphere of Colocasia esculenta. :: International Journal of Systematic and Evolutionary Microbiology. 2017 ; 巻 67, 番号 11. pp. 4572-4577.
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abstract = "A non-Streptomyces actinomycete, designated as strain S265T, was isolated from rhizosphere collected under an elephant ear plant (Colocasia esculenta) in Bangkok, Thailand. The taxonomic position of this strain was determined by a polyphasic approach. Strain S265T formed single globose spores on long, branching, aerial hyphae. It produced abundant aerial mycelium with green colour. The cell wall contained meso-diaminopimelic acid, and diagnostic whole-cell sugars were arabinose and galactose. Phosphatidylethanolamine and diphosphatidylglycerol were detected predominantly as polar lipids, whereas mycolic acids were not found. The major menaquinone was MK-9(H4), and principal cellular fatty acids were C15: 1 B, iso-C16: 1 H, anteiso-C15: 0 and C15: 0 2-OH. The DNA G+C content was 69 mol{\%}. According to phylogenetic analysis, strain S265T was clustered with Saccharomonospora glauca K62T (98.1{\%}) and Saccharomonospora viridis DSM 43017T (97.1{\%}) despite its 16S rRNA gene sequence showing the highest similarity value to that of Saccharomonospora azurea NA-128T (98.6{\%}). DNA–DNA relatedness values between strain S265T and the closely related strains were in the range of 7–50{\%}, thus strengthening the evidence derived from the polyphasic study that strain S265T represents a novel species within the genus Saccharomonospora, for which the name Saccharomonospora colocasiae sp. nov. is proposed. The type strain is S265T (=TBRC 7235T=NBRC 112945T).",
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AU - Wattanasuepsin, Watsapon

AU - Intra, Bungonsiri

AU - Take, Akira

AU - Inahashi, Yuki

AU - Euanorasetr, Jirayut

AU - Ōmura, Satoshi

AU - Matsumoto, Atsuko

AU - Panbangred, Watanalai

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