Sarpeptins A and B, Lipopeptides Produced by Streptomyces sp. KO-7888 Overexpressing a Specific SARP Regulator

Wilaiwan Koomsiri, Yuki Inahashi*, Kantinan Leetanasaksakul, Kazuro Shiomi, Yō Ko Takahashi, Satoshi Ō Mura, Markiyan Samborskyy, Peter F. Leadlay, Pakorn Wattana-Amorn, Arinthip Thamchaipenet, Takuji Nakashima

*この研究の対応する著者

研究成果査読

5 被引用数 (Scopus)

抄録

Whole genome analysis of Streptomyces sp. KO-7888 has revealed various pathway-specific transcriptional regulatory genes associated with silent biosynthetic gene clusters. A Streptomyces antibiotic regulatory protein gene, speR, located adjacent to a novel nonribosomal peptide synthetase (NRPS) gene cluster, was overexpressed in the wild-type strain. The resulting recombinant strain of Streptomyces sp. KO-7888 produced two new lipopeptides, sarpeptins A and B. Their structures were elucidated by high-resolution electrospray ionization mass spectrometry, NMR analysis, and the advanced Marfey's method. The distinct modular sections of the corresponding NRPS biosynthetic gene cluster were characterized, and the assembly line for production of the lipopeptide chain was proposed.

本文言語English
ページ(範囲)2144-2151
ページ数8
ジャーナルJournal of Natural Products
82
8
DOI
出版ステータスPublished - 2019 8 23
外部発表はい

ASJC Scopus subject areas

  • 分析化学
  • 分子医療
  • 薬理学
  • 薬科学
  • 創薬
  • 補完代替医療
  • 有機化学

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