TY - JOUR
T1 - Sarpeptins A and B, Lipopeptides Produced by Streptomyces sp. KO-7888 Overexpressing a Specific SARP Regulator
AU - Koomsiri, Wilaiwan
AU - Inahashi, Yuki
AU - Leetanasaksakul, Kantinan
AU - Shiomi, Kazuro
AU - Takahashi, Yō Ko
AU - Ō Mura, Satoshi
AU - Samborskyy, Markiyan
AU - Leadlay, Peter F.
AU - Wattana-Amorn, Pakorn
AU - Thamchaipenet, Arinthip
AU - Nakashima, Takuji
N1 - Funding Information:
W.K. has received a Royal Golden Jubilee Ph.D. scholarship (Grant No. PHD/0205/2553), Thailand Research Fund. Y.I. was supported by a Kitasato University Research Grant for Young Researchers. This work was financially supported by JSPS-NRCT under the Japan-Thailand Research Cooperative Program; the Institute for Fermentation, Osaka (IFO), Japan; Omics Center for Agriculture, Bioresources, Food and Health, Kasetsart University (OmiKU), Bangkok, Thailand; and the Herchel Smith Chair of Biochemistry Fund, University of Cambridge, U.K.
Publisher Copyright:
Copyright © 2019 American Chemical Society and American Society of Pharmacognosy.
PY - 2019/8/23
Y1 - 2019/8/23
N2 - Whole genome analysis of Streptomyces sp. KO-7888 has revealed various pathway-specific transcriptional regulatory genes associated with silent biosynthetic gene clusters. A Streptomyces antibiotic regulatory protein gene, speR, located adjacent to a novel nonribosomal peptide synthetase (NRPS) gene cluster, was overexpressed in the wild-type strain. The resulting recombinant strain of Streptomyces sp. KO-7888 produced two new lipopeptides, sarpeptins A and B. Their structures were elucidated by high-resolution electrospray ionization mass spectrometry, NMR analysis, and the advanced Marfey's method. The distinct modular sections of the corresponding NRPS biosynthetic gene cluster were characterized, and the assembly line for production of the lipopeptide chain was proposed.
AB - Whole genome analysis of Streptomyces sp. KO-7888 has revealed various pathway-specific transcriptional regulatory genes associated with silent biosynthetic gene clusters. A Streptomyces antibiotic regulatory protein gene, speR, located adjacent to a novel nonribosomal peptide synthetase (NRPS) gene cluster, was overexpressed in the wild-type strain. The resulting recombinant strain of Streptomyces sp. KO-7888 produced two new lipopeptides, sarpeptins A and B. Their structures were elucidated by high-resolution electrospray ionization mass spectrometry, NMR analysis, and the advanced Marfey's method. The distinct modular sections of the corresponding NRPS biosynthetic gene cluster were characterized, and the assembly line for production of the lipopeptide chain was proposed.
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U2 - 10.1021/acs.jnatprod.9b00074
DO - 10.1021/acs.jnatprod.9b00074
M3 - Article
C2 - 31381320
AN - SCOPUS:85071000568
SN - 0163-3864
VL - 82
SP - 2144
EP - 2151
JO - Journal of Natural Products
JF - Journal of Natural Products
IS - 8
ER -