@article{fa57de1684964421b2a9a5a6e25182c4,
title = "Secretion of biologically-active human interferon-β by Bacillus subtilis",
abstract = "Human interferon-β (hIFN-β) was used as a heterologous model protein to investigate the effects of the Bacillus subtilis AmyE propeptide and co-expression of PrsA in enhancing the secretion of heterologous proteins in B. subtilis. Secretion and activity of hIFN-β with AmyE propeptide increased by more than four-fold compared to that without AmyE propeptide. Moreover, under conditions of co-expressed PrsA, the secretion production and activity of hIFN-β with AmyE propeptide increased by more than 1. 5-fold. AmyE propeptide and co-expression of PrsA thus have an additive effect on enhancing the production of the hIFN-β in B. subtilis.",
keywords = "Bacillus subtilis, Human interferon-β, Propeptide, Protein secretion, PrsA",
author = "Hiroshi Kakeshita and Yasushi Kageyama and Keiji Endo and Masatoshi Tohata and Katsutoshi Ara and Katsuya Ozaki and Kouji Nakamura",
note = "Funding Information: Acknowledgments This work is the subproject, {\textquoteleft}Development of a Technology for Creation of a Host Cell{\textquoteright} included within the industrial technology project, {\textquoteleft}Development of a Generic Technology for Production Process Starting Productive Function{\textquoteright} of the Ministry of Economy, Trade and Industry, entrusted by the New Energy and Industrial Technology Development Organization (NEDO), Japan. Copyright: Copyright 2012 Elsevier B.V., All rights reserved.",
year = "2011",
month = sep,
doi = "10.1007/s10529-011-0636-2",
language = "English",
volume = "33",
pages = "1847--1852",
journal = "Biotechnology Letters",
issn = "0141-5492",
publisher = "Springer Netherlands",
number = "9",
}