抄録
Microbial degradation of carbazole (CA), a model of hard-removal heterocyclic nitrogen compounds contained in petroleum oil, was examined using Sphingomonas sp. CDH-7 isolated from a soil sample by screening for CA-as-similating microorganisms. CDH-7 used CA as a sole source of carbon and nitrogen, and metabolized CA to ammonia via anthranilic acid as an intermediate product. When CDH-7 was cultivated in the medium containing CA at the concentration of 500 mg/1 (2.99 mM), CA was completely degraded within 50 h. By the reaction with the resting cells of CDH-7, 500 mg/1 of CA was completely degraded within 4 h, with 1.64 mM of ammonia accumulated in the reaction mixture. When CA was added at the concentration of 100 mg/1 (0.599 mM) periodically to the reaction mixture ten times, 925 mg/1 (5.54 mM) of CA was degraded within 48 h by the resting cells, and 4.50 mM of ammonia was accumulated in the reaction mixture with a 75.1% molar conversion yield based on total CA added. The resting cells could almost completely degrade CA in a two-liquid-phase system which consists of water and organic solvent, even in the presence of 20% (v/v) isooctane, n-hexane, cyciohexane, and kerosene as a model petroleum oil. In the presence of an organic solvent system such as 20% (v/v)p-xylene, toluene, and heptanol, however, CA degradation yields decreased.
| 元の言語 | English |
|---|---|
| ページ（範囲） | 1563-1568 |
| ページ数 | 6 |
| ジャーナル | Bioscience, Biotechnology and Biochemistry |
| 巻 | 63 |
| 発行部数 | 9 |
| 出版物ステータス | Published - 1999 9 |
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ASJC Scopus subject areas
- Food Science
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Biotechnology
- Chemistry (miscellaneous)
- Applied Microbiology and Biotechnology
- Bioengineering
これを引用
Selective and continuous degradation of carbazole contained in petroleum oil by resting cells of Sphingomonas sp. CDH-7. / Kirimura, Kotaro; Nakagawa, Hiroyuki; Tsuji, Kenji; Matsuda, Kazuya; Kurane, Ryuichiro; Usami, Shoji.
：: Bioscience, Biotechnology and Biochemistry, 巻 63, 番号 9, 09.1999, p. 1563-1568.研究成果: Article
}
TY - JOUR
T1 - Selective and continuous degradation of carbazole contained in petroleum oil by resting cells of Sphingomonas sp. CDH-7
AU - Kirimura, Kotaro
AU - Nakagawa, Hiroyuki
AU - Tsuji, Kenji
AU - Matsuda, Kazuya
AU - Kurane, Ryuichiro
AU - Usami, Shoji
PY - 1999/9
Y1 - 1999/9
N2 - Microbial degradation of carbazole (CA), a model of hard-removal heterocyclic nitrogen compounds contained in petroleum oil, was examined using Sphingomonas sp. CDH-7 isolated from a soil sample by screening for CA-as-similating microorganisms. CDH-7 used CA as a sole source of carbon and nitrogen, and metabolized CA to ammonia via anthranilic acid as an intermediate product. When CDH-7 was cultivated in the medium containing CA at the concentration of 500 mg/1 (2.99 mM), CA was completely degraded within 50 h. By the reaction with the resting cells of CDH-7, 500 mg/1 of CA was completely degraded within 4 h, with 1.64 mM of ammonia accumulated in the reaction mixture. When CA was added at the concentration of 100 mg/1 (0.599 mM) periodically to the reaction mixture ten times, 925 mg/1 (5.54 mM) of CA was degraded within 48 h by the resting cells, and 4.50 mM of ammonia was accumulated in the reaction mixture with a 75.1% molar conversion yield based on total CA added. The resting cells could almost completely degrade CA in a two-liquid-phase system which consists of water and organic solvent, even in the presence of 20% (v/v) isooctane, n-hexane, cyciohexane, and kerosene as a model petroleum oil. In the presence of an organic solvent system such as 20% (v/v)p-xylene, toluene, and heptanol, however, CA degradation yields decreased.
AB - Microbial degradation of carbazole (CA), a model of hard-removal heterocyclic nitrogen compounds contained in petroleum oil, was examined using Sphingomonas sp. CDH-7 isolated from a soil sample by screening for CA-as-similating microorganisms. CDH-7 used CA as a sole source of carbon and nitrogen, and metabolized CA to ammonia via anthranilic acid as an intermediate product. When CDH-7 was cultivated in the medium containing CA at the concentration of 500 mg/1 (2.99 mM), CA was completely degraded within 50 h. By the reaction with the resting cells of CDH-7, 500 mg/1 of CA was completely degraded within 4 h, with 1.64 mM of ammonia accumulated in the reaction mixture. When CA was added at the concentration of 100 mg/1 (0.599 mM) periodically to the reaction mixture ten times, 925 mg/1 (5.54 mM) of CA was degraded within 48 h by the resting cells, and 4.50 mM of ammonia was accumulated in the reaction mixture with a 75.1% molar conversion yield based on total CA added. The resting cells could almost completely degrade CA in a two-liquid-phase system which consists of water and organic solvent, even in the presence of 20% (v/v) isooctane, n-hexane, cyciohexane, and kerosene as a model petroleum oil. In the presence of an organic solvent system such as 20% (v/v)p-xylene, toluene, and heptanol, however, CA degradation yields decreased.
KW - Biodegradation
KW - Carbazole
KW - Heterocyclic nitrogen compound
KW - Resting cell reaction
KW - Sphingomonas sp.
UR - http://www.scopus.com/inward/record.url?scp=0033193959&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033193959&partnerID=8YFLogxK
M3 - Article
C2 - 10540744
AN - SCOPUS:0033193959
VL - 63
SP - 1563
EP - 1568
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
SN - 0916-8451
IS - 9
ER -