Among 200 strains of marine bluegreen algae isolated from the coastal areas of Japan, the marine blue-green alga Synechococcus sp. NKBG 040607 excreted glutamate at the highest rate, 82.6% of total amino acids production being glutamate. Synechococcus sp. NKBG 40607 was immobilized in calcium alginate gel. Glutamate production by immobilized cells was double that of native cells. Maximal glutamate production (25 μg/cm3 gel per day) of the immobilized cells was observed under a light intensity of 144 μEinstein/m2 per second at a cell concentration of 7.5 mg dry cells/cm3 gel. Immobilized cells of Synechococcus sp. can use nitrate as a nitrogen source. Immobilized marine Synechococcus sp. produced 0265 mg/cm3 gel of glutamate for 7 days in the presence of chloramphenicol.
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