Semaphorin3A signaling mediated by fyn-dependent tyrosine phosphorylation of collapsin response mediator protein 2 at tyrosine 32

Yutaka Uchida, Toshio Ohshima, Naoya Yamashita, Miyuki Ogawara, Yukio Sasaki, Fumio Nakamura, Yoshio Goshima*

*この研究の対応する著者

研究成果: Article査読

47 被引用数 (Scopus)

抄録

Collapsin response mediator protein 2 (CRMP2) is an intracellular protein that mediates signaling of Semaphorin3A (Sema3A), a repulsive axon guidance molecule. Fyn, a Src-type tyrosine kinase, is involved in the Sema3A signaling. However, the relationship betweenCRMP2and Fyn in this signaling pathway is still unknown. In our research, we demonstrated that Fyn phosphorylated CRMP2 at Tyr32 residues in HEK293T cells. Immunohistochemical analysis using a phospho-specific antibody at Tyr32 of CRMP showed that Tyr32-phosphorylated CRMP was abundant in the nervous system, including dorsal root ganglion neurons, the molecular and Purkinje cell layer of adult cerebellum, and hippocampal fimbria. Overexpression of a nonphosphorylated mutant (Tyr32 to Phe32) of CRMP2 in dorsal root ganglion neurons interfered with Sema3A-induced growth cone collapse response. These results suggest that Fyn-dependent phosphorylation of CRMP2 at Tyr32 is involved in Sema3A signaling.

本文言語English
ページ(範囲)27393-27401
ページ数9
ジャーナルJournal of Biological Chemistry
284
40
DOI
出版ステータスPublished - 2009 10月 2

ASJC Scopus subject areas

  • 生化学
  • 分子生物学
  • 細胞生物学

フィンガープリント

「Semaphorin3A signaling mediated by fyn-dependent tyrosine phosphorylation of collapsin response mediator protein 2 at tyrosine 32」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル