Senescence marker protein-30 as a novel antiaging molecule

Dongyun Feng, Yoshitaka Kondo, Akihito Ishigami, Masashi Kuramoto, Takeo Machida, Naoki Maruyama*

*この研究の対応する著者

研究成果: Article査読

26 被引用数 (Scopus)

抄録

Senescence marker protein-30 (SMP30), composed of 299 amino acids, has an approximate molecular mass of 32-34 kDa and has a pI 4.9 in charge. The amino acid alignment from various animal species revealed a highly conserved structure. SMP30 has an enzyme activity hydrolyzing sarin, soman, and tabun, known as lethal toxic nerve chemicals. We analyzed the organophosphatase activity of SMP30 using DFP as a substrate. This DFPase activity is revealed in a dose-dependent manner in the presence of magnesium ions. We investigated the intracellular localization of SMP30. It is localized in both the cytoplasm and nucleus. To confirm the presence of SMP30 in the nucleus, we prepared nuclear and cytoplasmic extracts from isolated cultured hepatocytes. Western blotting showed that SMP30 was detected in both extracts. Because the expression is reduced by carbon tetrachloride, one can speculate that the expression is modulated by oxidative stress increased with aging.

本文言語English
ページ(範囲)360-364
ページ数5
ジャーナルAnnals of the New York Academy of Sciences
1019
DOI
出版ステータスPublished - 2004
外部発表はい

ASJC Scopus subject areas

  • 神経科学(全般)
  • 生化学、遺伝学、分子生物学(全般)
  • 科学史および科学哲学

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