Sensitive fluorescence-activated sorting of microdroplets containing subcelluar structures by thermoreversible gelation polymer

H. Okada, A. Iguchi, R. Iizuka, Donghyun Yoon, Tetsushi Sekiguchi, Shuichi Shoji*, T. Funatsu

*この研究の対応する著者

研究成果: Conference contribution

2 被引用数 (Scopus)

抄録

We have developed a microfluidic-based fluorescence-activated droplet sorting system using a thermoreversible gelation polymer (TGP) as a switching material. Compared with the conventional fluorescence-activated cell sorter (FACS), TGP-based fluorescence-activated droplet sorting can be performed using a relatively simple optical setup and enables a highly sensitive detection of the fluorescent target [1, 2]. Using this system, we successfully demonstrated the quick and sufficient isolation of a fluorescently labeled subcellular structure (human artificial chromosome; HAC) from the crude cell homogenate. The results presented here constitute the first demonstration of droplet sorting using TGP. This droplet-based microfluidics platform can complement the conventional techniques in proteomics.

本文言語English
ホスト出版物のタイトル18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
出版社Chemical and Biological Microsystems Society
ページ1259-1261
ページ数3
ISBN(印刷版)9780979806476
出版ステータスPublished - 2014
イベント18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States
継続期間: 2014 10 262014 10 30

Other

Other18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
国/地域United States
CitySan Antonio
Period14/10/2614/10/30

ASJC Scopus subject areas

  • 制御およびシステム工学

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