In this paper, we describe the development of a microfluidic centrifuge with two inlets and two outlets potentially capable of rapidly separating nanoparticles and nanovesicles. Compared with the microfluidic centrifuge with a single inlet and outlet, the 2 × 2 microfluidic centrifuge gives improved centrifugation performance by increasing momentum flux transfer, angular velocity, and centrifugal acceleration. The center of flow rotation and the symmetry of the horizontal velocity in the microchamber were examined numerically. On the basis of the determined maximum velocity, the angular velocity and centrifugal acceleration were also evaluated. The centrifugation time of three different nanoparticles was examined by calculating the time when the nanoparticles left the microchamber for the first time. For visual observation and quantitative measurement of nanoparticle centrifugation, a 2microfluidic centrifuge was fabricated and the experimental results demonstrate similar physical behavior to those of a mechanical centrifuge. On the basis of a comparison of the centrifugation time of two different nanoparticle populations of 300 and 700nm in diameter, we propose that nanoparticles of different sizes can be physically separated by time under a range of inlet volume flow rates.
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