Serial DNA immobilization in micro- and extended nanospace channels

Björn Renberg, Kae Sato, Kazuma Mawatari, Naokazu Idota, Takehiko Tsukahara, Takehiko Kitamori*

*この研究の対応する著者

研究成果: Article査読

27 被引用数 (Scopus)

抄録

That focused arrays, even with a small set of ligands, provide more data than single point experiments is well established in the DNA microarray research field, but microarray technology has yet to be transferred to fused silica microchips. Fused silica microchips have several attractive features such as stability to pressure, solvents, acids and bases, and can be fabricated with minute dimensions, making them good candidates for nanofluidic research. However, due to harsh bonding conditions, DNA ligands must be immobilized after fabrication, thus preventing standard microarray spotting techniques from being used. In this paper, we provide tools for serial DNA immobilization in fused silica microchips using UV. We report the synthesis of a new UV-linker which was used to covalently couple functional DNA oligos to the inside of channels in fused silica microchips. With some simple modifications to our mask aligner, we were able to transfer OHP mask patterns, which allows the creation of basically any pattern in the channels. The functionality of the oligos was measured through the binding of fluorophore-labeled complementary target oligos. We examined parameters influencing DNA immobilization, and carry-over between spots after consecutive immobilizations inside the same channel. We also report the first successful multiple immobilizations of functional DNA oligos inside single channels of extended nanospace depth (460 nm).

本文言語English
ページ(範囲)1517-1523
ページ数7
ジャーナルLab on a Chip - Miniaturisation for Chemistry and Biology
9
11
DOI
出版ステータスPublished - 2009
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 化学 (全般)
  • バイオエンジニアリング
  • 生体医工学

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