Serial DNA relay in DNA logic gates by electrical fusion and mechanical splitting of droplets

Hiroki Yasuga, Kosuke Inoue, Ryuji Kawano, Masahiro Takinoue, Toshihisa Osaki, Koki Kamiya, Norihisa Miki, Shoji Takeuchi

研究成果: Article

2 引用 (Scopus)

抜粋

DNA logic circuits utilizing DNA hybridization and/or enzymatic reactions have drawn increasing attention for their potential applications in the diagnosis and treatment of cellular diseases. The compartmentalization of such a system into a microdroplet considerably helps to precisely regulate local interactions and reactions between molecules. In this study, we introduced a relay approach for enabling the transfer of DNA from one droplet to another to implement multi-step sequential logic operations. We proposed electrical fusion and mechanical splitting of droplets to facilitate the DNA flow at the inputs, logic operation, output, and serial connection between two logic gates. We developed Negative-OR operations integrated by a serial connection of the OR gate and NOT gate incorporated in a series of droplets. The four types of input defined by the presence/absence of DNA in the input droplet pair were correctly reflected in the readout at the Negative-OR gate. The proposed approach potentially allows for serial and parallel logic operations that could be used for complex diagnostic applications.

元の言語English
記事番号e0180876
ジャーナルPloS one
12
発行部数7
DOI
出版物ステータスPublished - 2017 7 1
外部発表Yes

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ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

これを引用

Yasuga, H., Inoue, K., Kawano, R., Takinoue, M., Osaki, T., Kamiya, K., Miki, N., & Takeuchi, S. (2017). Serial DNA relay in DNA logic gates by electrical fusion and mechanical splitting of droplets. PloS one, 12(7), [e0180876]. https://doi.org/10.1371/journal.pone.0180876