Determination of protein charges under physiological conditions by field effect transistors (FETs) is challenging because of the screening effect by solution counter ions. We showed that sugar-chain-modified FETs can detect proteins under physiological conditions. Protein charges are detectable on the gate interface by applying sugar chains as receptors: these sugar chains are short and highly flexible. Threshold voltage shifts caused by adsorption of SSA lectin from Sambucus sieboldiana onto sialylglycan-modified FET showed a linear relationship with SSA concentrations under physiological conditions.
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