抄録
A non-magnetic mutant, NMA61, of the magnetic bacterium Magnetospirillum magneticum AMB-1 was generated by transposon mutagenesis to identify genes involved in magnetosome synthesis. The genomic region of NMA61 interrupted by a Mini-Tn5 transposon was analyzed. The transposon was inserted in an open reading frame (ORF) coding for a periplasmic transport binding protein kinase gene homologue. Three adjacent ORFs and a promoter were identified upstream, indicating that the sequences comprised an operon. Phenotype characterizations showed that the growth inhibition imposed by the exogenous non-assimilable iron chelator nitrilotriacetate was relieved in wild type but not in NMA61, by the addition of the isolated wild type siderophore. Higher concentration of siderophores accumulated in the culture medium of NMA61 than in wild type. These data suggest that the interrupted periplasmic transport binding protein kinase gene homologue is required for siderophore transport into M. magneticum AMB-1.
本文言語 | English |
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ページ(範囲) | 852-857 |
ページ数 | 6 |
ジャーナル | Biochemical and Biophysical Research Communications |
巻 | 323 |
号 | 3 |
DOI | |
出版ステータス | Published - 2004 10月 22 |
外部発表 | はい |
ASJC Scopus subject areas
- 生物理学
- 生化学
- 分子生物学
- 細胞生物学