Simple and rapid CD4 testing based on large-field imaging system composed of microcavity array and two-dimensional photosensor

Tatsuya Saeki, Yuriko Sugamura, Masahito Hosokawa, Tomoko Yoshino, Tae kyu Lim, Manabu Harada, Tadashi Matsunaga, Tsuyoshi Tanaka*

*この研究の対応する著者

研究成果: Article査読

6 被引用数 (Scopus)

抄録

This study presents a novel method for CD4 testing based on one-shot large-field imaging. The large-field imaging system was fabricated by a microcavity array and a two-dimensional (2D) photosensor within the desk-top-sized instrument. The microcavity array was employed to separate leukocytes from whole blood based on differences in the size of leukocytes and other blood cells. The large-field imaging system with lower side irradiation enabled acquisition of cell signatures with high signal-to-noise ratio, because the metallic substrate of the microcavity array obstructed excessive excitation light. In this setting, dual-color imaging of CD4+ and CD8+ T cells was achieved within the entire image area (64mm2) in 2s. The practical performance of the large-field imaging system was demonstrated by determining the CD4/CD8 ratio in a few microliter of control whole blood as small as those obtained by a finger prick. The CD4/CD8 ratios measured using the large-field imaging system correlated well with those measured by microscopic analysis. These results indicate that our proposed system provides a simple and rapid CD4 testing for the application of HIV/AIDS treatment.

本文言語English
ページ(範囲)350-355
ページ数6
ジャーナルBiosensors and Bioelectronics
67
DOI
出版ステータスPublished - 2015 5月 5
外部発表はい

ASJC Scopus subject areas

  • バイオテクノロジー
  • 生物理学
  • 生体医工学
  • 電気化学

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