Simple estimation of the differential absorption coefficient of P-700 in detergent-treated preparations

Kintake Sonoike, Sakae Katoh*

*この研究の対応する著者

研究成果: Article査読

16 被引用数 (Scopus)

抄録

The apparent differential absorption coefficient of P-700 in the thylakoid membranes and Photosystem I particles from the cyanobacterium Synechococcus elongatus was differently affected by various detergents. Triton X-100 and sodium dodecyl sulfate increased the differential absorption coefficient, whereas the absorption value was reduced by digitonin and octyl-d-glucoside. Irrespective of detergents used, however, variations of the differential absorption coefficient were well related to band shifts of chlorophyll a molecules at 690 nm. The band shift also affected, in opposite directions, the two bleaching bands at 680 nm and 700 nm of the P-700 difference spectrum. Thus the differential absorption coefficient of P-700 in detergent-treated preparations can be estimated by measuring the ratio of the 680 nm to 700 nm bands.

本文言語English
ページ(範囲)210-213
ページ数4
ジャーナルBBA - Bioenergetics
976
2-3
DOI
出版ステータスPublished - 1989 9月 28
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 細胞生物学

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