In this study, a simple method for quantitative PCR (qPCR) with a microfluidic device is proposed and evaluated. PCR was performed in a flow-through manner with a SYBR green containing PCR mixture. During and after the PCR operations, the fluorescence intensity was continuously monitored to obtain forward and inversed amplification curves. As a result of evaluation, quite linear calibration curve was obtained both with the forward and the inversed amplification curve.
|出版ステータス||Published - 2008 1月 1|
|イベント||12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States|
継続期間: 2008 10月 12 → 2008 10月 16
|Conference||12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008|
|City||San Diego, CA|
|Period||08/10/12 → 08/10/16|
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