Simple method for quantitative PCR using flow-through PCR device

T. Fukuba; M. Hiraga; A. Takamatsu; C. Provin; T. Yamamoto; T. Fujii

Simple method for quantitative PCR using flow-through PCR device

T. Fukuba, M. Hiraga, A. Takamatsu, C. Provin, T. Yamamoto, T. Fujii

先進理工学部

研究成果: Paper › 査読

抄録

In this study, a simple method for quantitative PCR (qPCR) with a microfluidic device is proposed and evaluated. PCR was performed in a flow-through manner with a SYBR green containing PCR mixture. During and after the PCR operations, the fluorescence intensity was continuously monitored to obtain forward and inversed amplification curves. As a result of evaluation, quite linear calibration curve was obtained both with the forward and the inversed amplification curve.

本文言語	English
ページ	1743-1745
ページ数	3
出版ステータス	Published - 2008 1 1
イベント	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States 継続期間: 2008 10 12 → 2008 10 16

Conference

Conference	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
国/地域	United States
City	San Diego, CA
Period	08/10/12 → 08/10/16

ASJC Scopus subject areas

化学工学（その他）
バイオエンジニアリング

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引用スタイル

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title = "Simple method for quantitative PCR using flow-through PCR device",

abstract = "In this study, a simple method for quantitative PCR (qPCR) with a microfluidic device is proposed and evaluated. PCR was performed in a flow-through manner with a SYBR green containing PCR mixture. During and after the PCR operations, the fluorescence intensity was continuously monitored to obtain forward and inversed amplification curves. As a result of evaluation, quite linear calibration curve was obtained both with the forward and the inversed amplification curve.",

keywords = "Flow-through polymerase chain reaction, Polydimethylsiloxane, Quantitative polymerase chain reaction",

author = "T. Fukuba and M. Hiraga and A. Takamatsu and C. Provin and T. Yamamoto and T. Fujii",

year = "2008",

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T1 - Simple method for quantitative PCR using flow-through PCR device

AU - Fukuba, T.

AU - Hiraga, M.

AU - Takamatsu, A.

AU - Provin, C.

AU - Yamamoto, T.

AU - Fujii, T.

PY - 2008/1/1

Y1 - 2008/1/1

N2 - In this study, a simple method for quantitative PCR (qPCR) with a microfluidic device is proposed and evaluated. PCR was performed in a flow-through manner with a SYBR green containing PCR mixture. During and after the PCR operations, the fluorescence intensity was continuously monitored to obtain forward and inversed amplification curves. As a result of evaluation, quite linear calibration curve was obtained both with the forward and the inversed amplification curve.

AB - In this study, a simple method for quantitative PCR (qPCR) with a microfluidic device is proposed and evaluated. PCR was performed in a flow-through manner with a SYBR green containing PCR mixture. During and after the PCR operations, the fluorescence intensity was continuously monitored to obtain forward and inversed amplification curves. As a result of evaluation, quite linear calibration curve was obtained both with the forward and the inversed amplification curve.

KW - Flow-through polymerase chain reaction

KW - Polydimethylsiloxane

KW - Quantitative polymerase chain reaction

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T2 - 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008

Y2 - 12 October 2008 through 16 October 2008

ER -

Simple method for quantitative PCR using flow-through PCR device

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Conference

ASJC Scopus subject areas

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