Simple method for quantitative PCR using flow-through PCR device

T. Fukuba, M. Hiraga, A. Takamatsu, C. Provin, T. Yamamoto, T. Fujii

研究成果: Paper査読

抄録

In this study, a simple method for quantitative PCR (qPCR) with a microfluidic device is proposed and evaluated. PCR was performed in a flow-through manner with a SYBR green containing PCR mixture. During and after the PCR operations, the fluorescence intensity was continuously monitored to obtain forward and inversed amplification curves. As a result of evaluation, quite linear calibration curve was obtained both with the forward and the inversed amplification curve.

本文言語English
ページ1743-1745
ページ数3
出版ステータスPublished - 2008 1 1
イベント12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States
継続期間: 2008 10 122008 10 16

Conference

Conference12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
国/地域United States
CitySan Diego, CA
Period08/10/1208/10/16

ASJC Scopus subject areas

  • 化学工学(その他)
  • バイオエンジニアリング

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