抄録
In this study, a simple method for quantitative PCR (qPCR) with a microfluidic device is proposed and evaluated. PCR was performed in a flow-through manner with a SYBR green containing PCR mixture. During and after the PCR operations, the fluorescence intensity was continuously monitored to obtain forward and inversed amplification curves. As a result of evaluation, quite linear calibration curve was obtained both with the forward and the inversed amplification curve.
本文言語 | English |
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ページ | 1743-1745 |
ページ数 | 3 |
出版ステータス | Published - 2008 1月 1 |
イベント | 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States 継続期間: 2008 10月 12 → 2008 10月 16 |
Conference
Conference | 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 |
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国/地域 | United States |
City | San Diego, CA |
Period | 08/10/12 → 08/10/16 |
ASJC Scopus subject areas
- 化学工学(その他)
- バイオエンジニアリング