Single cell analysis of the proinflammatory responses of mast cell by a real time secretion assay

Y. Shirasaki, A. Nakahara, N. Shimura, N. Suzuki, M. Yamagishi, J. Mizuno, S. Shoji, O. Ohara

研究成果: Conference contribution

1 引用 (Scopus)

抜粋

A real-time secretion monitoring assay from living single-cells was realized by total internal reflection fluorescence imaging with an optically optimized microwell array chip fabricated of an amorphous fluoropolymer, CYTOP on the basis of a fluorescence sandwich immunoassay. After individual mast cells were stimulated with lipopolysaccharide in the microwell array, their proinflammatory responses were monitored as cytokine secretion along time. mRNA levels of cytokines by single-cell quantitative PCR was also measured after the secretion assay. The results showed that each of stimulated mast cells is quantitatively and qualitatively distinguishable from each other in terms of cytokine secretion and their mRNA profile.

元の言語English
ホスト出版物のタイトル15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
ページ843-845
ページ数3
出版物ステータスPublished - 2011 12 1
イベント15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
継続期間: 2011 10 22011 10 6

出版物シリーズ

名前15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
2

Conference

Conference15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
United States
Seattle, WA
期間11/10/211/10/6

    フィンガープリント

ASJC Scopus subject areas

  • Control and Systems Engineering

これを引用

Shirasaki, Y., Nakahara, A., Shimura, N., Suzuki, N., Yamagishi, M., Mizuno, J., Shoji, S., & Ohara, O. (2011). Single cell analysis of the proinflammatory responses of mast cell by a real time secretion assay. : 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 (pp. 843-845). (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011; 巻数 2).