Single-cell observation of phagocytosis by human blood dendritic cells

Hiroshi Ishimoto, Katsunori Yanagihara*, Nobuko Araki, Hiroshi Mukae, Noriho Sakamoto, Koichi Izumikawa, Masafumi Seki, Yoshitsugu Miyazaki, Yoichi Hirakata, Yohei Mizuta, Kenji Yasuda, Shigeru Kohno


研究成果: Article査読

15 被引用数 (Scopus)


Time-lapse video microscopic observation is useful for analysis of cell biology, especially in rapid response of immune cells. Dendritic cells (DCs) have multiple functions in the immune system, and DCs in peripheral blood play an especially important role at the front line of infection. We have developed a time-lapse video microscopic method for the evaluation of single myeloid DCs (MDC-1) from human peripheral blood. MDC-1 displayed generalized plasma membrane ruffling and phagocytosis of Pseudomonas aeruginosa. The morphological changes of MDC-1 increased following stimulation with P. aeruginosa but not after stimulation with supernatant from a P. aeruginosa culture. The activation of these morphological changes in MDC-1 could be quantitatively analyzed using the time-lapse video microscopy. This novel system may be useful for the evaluation of rapid response with human immune cells against bacterial infection.

ジャーナルJapanese Journal of Infectious Diseases
出版ステータスPublished - 2008 8月 11

ASJC Scopus subject areas

  • 微生物学(医療)
  • 感染症


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