Single-cell real-time imaging of flow-induced hemolysis using high-speed microfluidic technology

Takanobu Yagi, S. Wakasa, N. Tokunaga, Y. Akimoto, M. Umezu

研究成果: Conference contribution

抄録

Understanding the mechanism of flow-induced blood cell damage, such as hemolysis and platelet activation, plays an important role for arterial diseases and artificial organs. This study for the first time demonstrates the visualization of flow-induced hemolysis in a single-cell real-time manner using high-speed microfluidic technology. Impinging microjets with a velocity of 1.5 m/s order at a nozzle exit were made in the Y- and T-shaped microchannel. The curved (r=10μm) and flat collision surface were compared. Porcine fresh erythrocytes were suspended in PBS at Ht=0.5%. Results showed that membrane failure was only observed in the Y-junction. These erythrocytes were initially elongated at the far region, and then longitudinally compressed in the near wall region due to the sharp adverse pressure gradient, whereas those in the T-junction released the membrane tension as the pressure difference per erythrocyte diminished. In the simulation of energy balance, it was found that the dominant force for the longitudinal compression was the pressure difference per erythrocyte. Such erythrocytes showed the sudden drop of elastic modulus, suggesting that the elongated spectrin network of erythrocyte was fragile for the compressive force and immediately broken by the impact force.

本文言語English
ホスト出版物のタイトルWorld Congress on Medical Physics and Biomedical Engineering
ホスト出版物のサブタイトルImage Processing, Biosignal Processing, Modelling and Simulation, Biomechanics
出版社Springer Verlag
ページ2337-2340
ページ数4
4
ISBN(印刷版)9783642038815
DOI
出版ステータスPublished - 2009
イベントWorld Congress on Medical Physics and Biomedical Engineering: Image Processing, Biosignal Processing, Modelling and Simulation, Biomechanics - Munich, Germany
継続期間: 2009 9 72009 9 12

出版物シリーズ

名前IFMBE Proceedings
番号4
25
ISSN(印刷版)1680-0737

Conference

ConferenceWorld Congress on Medical Physics and Biomedical Engineering: Image Processing, Biosignal Processing, Modelling and Simulation, Biomechanics
CountryGermany
CityMunich
Period09/9/709/9/12

ASJC Scopus subject areas

  • Bioengineering
  • Biomedical Engineering

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