Single nucleotide mismatch analysis using oligonucleotide probes synthesized on bacterial magnetic particle

Hiroyuki Ota, Atsushi Arakaki, Tsuyoshi Tanaka, Haruko Takeyama, Tadashi Matsunaga

研究成果: Article査読

2 被引用数 (Scopus)

抄録

An approach to analyze mismatches using short and specific oligonucleotide probes directly synthesized on bacterial magnetic particles (BMPs) by phosphoramidite methods was exploited. Approximately 126 molecules of 4-mer oligonucleotides/particle were synthesized on BMPs with high reaction efficiencies. Hybridization between FITC-labeled oligonucleotides and chemically synthesized oligonucleotides on BMPs was performed. Perfect matched and mismatched hybridizations were successfully discriminated by using the oligonucleotide probes on BMPs.

本文言語English
ページ(範囲)305-309
ページ数5
ジャーナルBiomolecular Engineering
20
4-6
DOI
出版ステータスPublished - 2003 7
外部発表はい

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Molecular Biology

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