Site-selective immobilization of streptavidin on enzymatically biotinylated bacterial magnetic particles

Yoshiaki Maeda, Tomoko Yoshino, Haruko Takeyama, Masaaki Takahashi, Harumi Ginya, Junko Asahina, Hideji Tajima, Tadashi Matsunaga

研究成果: Conference contribution

抄録

Biotinylated magnetic nanoparticles were constructed by displaying biotin acceptor peptide (BAP) on the surfaces of bacterial magnetic particles (BacMPs) synthesized by Magnetospirillum niagneticum AMB-1. Both BAP and green fluorescent protein (GFP) were fused to Mmsl3 that was isolated from BacMP membranes. The localization of the fusion protein, BAP-Mmsl3-GFP, was confirmed by fluorescence analysis. BacMPs that expressed B AP-Mms 13-GFP (BAP/GFP-BacMPs) were extracted from bacterial cells and incubated with biotin and Escherichia coli biotin ligase. The in vitro biotinylation of BAP/GFP-BacMPs was confirmed using alkaline phosphatase (ALP)-labeled streptavidin. The conjugation system developed in this study provides a method for producing biotin- or streptavidin-labeled magnetic nanoparticles without the use of a crosslinker reagent. Various functional materials can be immobilized site-selectively onto these uniquely designed BacMPs. By combining this site-selective biotinylation technology and protein display methodology, increasingly innovative and attractive magnetic nano-materials can be constructed.

本文言語English
ホスト出版物のタイトルFrom Biological Materials to Biomimetic Material Synthesis
ページ65-70
ページ数6
出版ステータスPublished - 2008 12 1
外部発表はい
イベント2008 MRS Spring Meeting - San Francisco, CA, United States
継続期間: 2008 3 242008 3 28

出版物シリーズ

名前Materials Research Society Symposium Proceedings
1094
ISSN(印刷版)0272-9172

Conference

Conference2008 MRS Spring Meeting
CountryUnited States
CitySan Francisco, CA
Period08/3/2408/3/28

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

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