SNP detection in transforming growth factor-β1 gene using bacterial magnetic particles

Hiroyuki Ota, Haruko Takeyama, Hideki Nakayama, Takahiko Katoh, Tadashi Matsunaga*

*この研究の対応する著者

研究成果: Article査読

31 被引用数 (Scopus)

抄録

A single nucleotide polymorphism (SNP) within the transforming growth factor-β1 (TGF-β1) gene was detected by hybridization-based method using bacterial magnetic particles (BMPs). TGF-β1 is commonly associated with a single base change resulting in a Leu10→Pro (T869→C) polymorphism and is a genetic marker for susceptibility to osteoporosis. Short (9 bases) and specific probes were designed to detect SNP in TGF-β1. Detection probes were immobilized on BMPs using cross-linking reagents. TGF-β1 PCR products (139 bp) were labeled with the fluorescent dye coumarin and hybridized with detection probes on BMPs. Complementary hybridized targets gave over four times higher fluorescent intensities, compared with one base mismatched hybridizations. The SNP genotype was successfully discriminated using this technique.

本文言語English
ページ(範囲)683-687
ページ数5
ジャーナルBiosensors and Bioelectronics
18
5-6
DOI
出版ステータスPublished - 2003 5 1
外部発表はい

ASJC Scopus subject areas

  • バイオテクノロジー
  • 生物理学
  • 生体医工学
  • 電気化学

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