Soft-X-ray damage to biological samples

H. Fujisaki*, S. Takahashi, H. Ohzeki, K. Sugisaki, H. Kondo, H. Nagata, H. Kato, S. Ishiwata

*この研究の対応する著者

    研究成果: Article査読

    9 被引用数 (Scopus)

    抄録

    X-ray damage to biological samples was investigated in the wavelength region of 2.7-5 nm, which overlaps the so-called 'water window', the wavelength range of 2.4- 4.3 nm usually used in X-ray microscopy. Yeast cells and myofibrils were chosen as representatives of whole cell samples and motile protein systems, respectively. The samples were exposed to X-rays using an apparatus composed mainly of a laser-plasma X-ray source, a Welter mirror condenser, and a sample cell. The yeast cells lost their dye exclusion ability when the X-ray flux was higher than 1 x 106 photons μm-2, while the myofibrils lost contractility when the X-ray flux was higher than 4 x 105 photons μm-2. These X-ray fluxes are lower than the nux required for the X-ray microscope observation of biological samples at. a resolution higher than that of light microscopes.

    本文言語English
    ページ(範囲)79-83
    ページ数5
    ジャーナルJournal of Microscopy
    182
    2
    出版ステータスPublished - 1996

    ASJC Scopus subject areas

    • 器械工学

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