Spermidine inhibits transient and stable ribosome subunit dissociation

So Umekage, Takuya Ueda*

*この研究の対応する著者

研究成果: Article査読

18 被引用数 (Scopus)

抄録

Recent light-scattering experiments and sucrose density gradient centrifugational analyses suggested that the 70S ribosome undergoes RRF- and EF-G-triggered transient subunit dissociation that is followed by IF3-induced stable dissociation. However, the experimental conditions did not include the ubiquitous cellular polyamine spermidine, which is required for efficient translation. We found that when spermidine was present, the transient dissociation was inhibited. Moreover, the published experiments used ribosome concentrations that were far lower than the physiological concentration. We found that when spermidine and higher ribosome concentrations were included in the experimental conditions, only very limited stable subunit dissociation was observed. These results suggest that neither transient nor stable dissociation occurs under physiological conditions applied here.

本文言語English
ページ(範囲)1222-1226
ページ数5
ジャーナルFEBS Letters
580
5
DOI
出版ステータスPublished - 2006 2月 20
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 構造生物学
  • 生化学
  • 分子生物学
  • 遺伝学
  • 細胞生物学

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