Spindle pole body components are reorganized during fission yeast meiosis

Midori Ohta, Masamitsu Sato*, Masayuki Yamamoto

*この研究の対応する著者

研究成果: Article査読

32 被引用数 (Scopus)

抄録

During meiosis, the centrosome/spindle pole body (SPB) must be regulated in a manner distinct from that of mitosis to achieve a specialized cell division that will produce gametes. In this paper, we demonstrate that several SPB components are localized to SPBs in a meiosis-specific manner in the fission yeast Schizosaccharomyces pombe. SPB components, such as Cut12, Pcp1, and Spo15, which stay on the SPB during the mitotic cell cycle, disassociate from the SPB during meiotic prophase and then return to the SPB immediately before the onset of meiosis I. Interestingly, the polo kinase Plo1, which normally localizes to the SPB during mitosis, is excluded from them in meiotic prophase, when meiosis-specific, horse-tail nuclear movement occurs. We found that exclusion of Plo1 during this period was essential to properly remodel SPBs, because artificial targeting of Plo1 to SPBs resulted in an overduplication of SPBs. We also found that the centrin Cdc31 was required for meiotic SPB remodeling. Thus Plo1 and a centrin play central roles in the meiotic SPB remodeling, which is essential for generating the proper number of meiotic SPBs and, thereby provide unique characteristics to meiotic divisions.

本文言語English
ページ(範囲)1799-1811
ページ数13
ジャーナルMolecular biology of the cell
23
10
DOI
出版ステータスPublished - 2012 5月 15
外部発表はい

ASJC Scopus subject areas

  • 分子生物学
  • 細胞生物学

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