Spontaneous oscillation of tension and sarcomere length in skeletal myofibrils. Microscopic measurement and analysis

T. Anazawa*, K. Yasuda, S. Ishiwata

*この研究の対応する著者

研究成果査読

51 被引用数 (Scopus)

抄録

We have devised a simple method for measuring tension development of single myofibrils by micromanipulation with a pair of glass micro-needles. The tension was estimated from the deflection of a flexible needle under an inverted phase-contrast microscope equipped with an image processor, so that the tension development is always accompanied by the shortening of the myofibril (auxotonic condition) in the present setup. The advantage of this method is that the measurement of tension (1/30 s for time resolution and about 0.05 micrograms for accuracy of tension measurement; 0.05 microns as a spatial resolution for displacement of the micro-needle) and the observation of sarcomere structure are possible at the same time, and the technique to hold myofibrils, even single myofibrils, is very simple. This method has been applied to study the tension development of glycerinated skeletal myofibrils under the condition where spontaneous oscillation of sarcomeres is induced, i.e., the coexistence of MgATP, MgADP and inorganic phosphate without free Ca2+. Under this condition, we found that the tension of myofibrils spontaneously oscillates accompanied by the oscillation of sarcomere length with a main period of a few seconds; the period was lengthened and shortened with stretch and release of myofibrils. A possible mechanism of the oscillation is discussed.

本文言語English
ページ(範囲)1099-1108
ページ数10
ジャーナルBiophysical Journal
61
5
DOI
出版ステータスPublished - 1992

ASJC Scopus subject areas

  • 生物理学

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