Spontaneous oscillatory contraction without regulatory proteins in actin filament-reconstituted fibers

Hideaki Fujita, Shin'ichi Ishiwata

    研究成果: Article査読

    38 被引用数 (Scopus)

    抄録

    Skinned skeletal and cardiac muscle fibers exhibit spontaneous oscillatory contraction (SPOC) in the presence of MgATP, MgADP, and inorganic phosphate (P(i)), but the molecular mechanism underlying this phenomenon is not yet clear. We have investigated the role of regulatory proteins in SPOC using cardiac muscle fibers of which the actin filaments had been reconstituted without tropomyosin and troponin, according to a previously reported method (Fujita et al., 1996. Biophys. J. 71:2307-2318). That is, thin filaments in glycerinated cardiac muscle fibers were selectively removed by treatment with gelsolin. Then, by adding exogenous actin to these thin filament-free cardiac muscle fibers under polymerizing conditions, actin filaments were reconstituted. The actin filament-reconstituted cardiac muscle fibers generated active tension in a Ca2+-insensitive manner because of the lack of regulatory proteins. Herein we have developed a new solvent condition under which SPOC occurs, even in actin filament-reconstituted fibers: the coexistence of 2,3-butanedione 2-monoxime (BDM), a reversible inhibitor of actomyosin interactions, with MgATP, MgADP and P(i). The role of BDM in the mechanism of SPOC in the actin filament-reconstituted fibers was analogous to that of the inhibitory function of the tropomyosin-troponin complex (- Ca2+) in the control fibers. The present results suggest that SPOC is a phenomenon that is intrinsic to the actomyosin motor itself.

    本文言語English
    ページ(範囲)1439-1445
    ページ数7
    ジャーナルBiophysical Journal
    75
    3
    出版ステータスPublished - 1998 9

    ASJC Scopus subject areas

    • Biophysics

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