Stabilization of mast cells by heme oxygenase-1: An anti-inflammatory role

Rina Takamiya, Makoto Murakami, Mayumi Kajimura, Nobuhito Goda, Nobuya Makino, Yoshihiro Takamiya, Tokio Yamaguchi, Yuzuru Ishimura, Nobumichi Hozumi, Makoto Suematsu*


研究成果: Article査読

54 被引用数 (Scopus)


This study examined the role of bilirubin in heme oxygenase (HO)-1-mediated amelioration of mast cell (MC)-elicited inflammatory responses. Pretreatment of rats with an intraperitoneal injection of hemin, an inducer of HO-1, evolved a marked induction of the enzyme in MCs. Intravital videomicroscopy revealed that hemin pretreatment attenuated compound 48/80-elicited degranulation, of MCs and resultant leukocyte adhesion in venules. Superfusion with biliverdin or bilirubin, but not with carbon monoxide (CO), another product of the HO reaction, mimicked suppressive actions of the HO-1 induction on both the cell degranulation and leukocyte adhesion elicited by the stimulus, suggesting a requirement of the enzyme reaction to generate bilirubin in the inhibitory mechanisms. Such MC-desensitizing actions of bilirubin were observed in primary-cultured MCs and reproduced irrespective of the choice of stimuli, such as compound 48/80, calcium ionophore, and anti-IgE serum. Furthermore, MC-stabilizing effects of HO-1 were reproduced by the gene transfection of the enzyme into mastocytoma cell line RBL2H3. These results suggest that bilirubin generated through HO-1 serves as an anti-inflammatory substance that desensitizes MCs and ameliorates leukocyte recruitment.

ジャーナルAmerican Journal of Physiology - Heart and Circulatory Physiology
3 52-3
出版ステータスPublished - 2002

ASJC Scopus subject areas

  • 生理学
  • 循環器および心血管医学
  • 生理学(医学)


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