Stage-dependent distribution of calreticulin in oocytes of the frog, Rana rugosa

Masahisa Nakamura*, Kiichirou Kawano, Yasuyuki Kondo, Ikuo Miura, Akihiko Kashiwagi, Satoshi Yamamoto

*この研究の対応する著者

研究成果査読

抄録

We studied immunohistologically the distribution of a Ca2+-binding protein, calreticulin (CLT), at different stages of growing oocytes in the frog, Rana rugosa. Northern blot analysis showed that the CLT gene expression in gonads of metamorphosing tadpoles was low, but was extremely strong in the ovary, but not in the testis, of 2-month post-metamorphosis frogs, followed by decline to a lower level in adult frogs. On the contrary, the β-actin gene expression did not increase in the same ovary. As for the distribution of CLT protein, a weak immunostaining was observed in indifferent gonads of tadpoles at stage I. The CLT distribution in oocytes from stage A to F was stage-dependent. In addition, Western blot analysis revealed that the CLT level was low in gonads of tadpoles at stage I, but increased at stage XVI. It still increased in the ovary of frogs 2 months after metamorphosis, and then dropped to a lower level in adult frogs. The results indicate that CLT gene expression occurs in the early stage of growing oocytes, and that CLT is synthesized actively in oocytes in the ovary of frogs after metamorphosis. Based on these findings, the role of CLT is discussed.

本文言語English
ページ(範囲)935-940
ページ数6
ジャーナルZoological Science
14
6
出版ステータスPublished - 1997 12
外部発表はい

ASJC Scopus subject areas

  • 動物科学および動物学

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