Stepwise pattern modification of neuronal network during cultivation using photo-thermal etching of agarose architecture

Yoshihiro Sugio, Hiroyuki Moriguchi, Ikurou Suzuki, Tomoyuki Kaneko, Kenji Yasuda, Yasuhiko Jimbo

研究成果: Conference contribution

抄録

We have developed a new type of on-chip cell cultivation system using an agarose microchamber (AMC) array and a photo-thermal etching method, thus enabling topographical control of neuronal network pattern step-by-step during cell cultivation. By using photo-thermal etching (micro melting) method, the number of microtunnel connecting microchambers can be easily increased, even during cell cultivation, according to the progress of the neuronal network formation. To demonstrate the capability of this system for topographical control of network formation, we cultured hippocampal neurons in this AMC array. We found that the cells in microchambers made fiber connections through microtunnels. Furthermore the cells even made fiber connections through additional microtunnels fabricated during cultivation by photo-thermal etching. The results showed that the photo-thermal etching could be used during cultivation without damaging cells.

本文言語English
ホスト出版物のタイトルDigest of Papers - Microprocesses and Nanotechnology 2003 - 2003 International Microprocesses and Nanotechnology Conference, MNC 2003
出版社Institute of Electrical and Electronics Engineers Inc.
ページ322-323
ページ数2
ISBN(電子版)4891140402, 9784891140403
DOI
出版ステータスPublished - 2003
外部発表はい
イベントInternational Microprocesses and Nanotechnology Conference, MNC 2003 - Tokyo, Japan
継続期間: 2003 10月 292003 10月 31

Other

OtherInternational Microprocesses and Nanotechnology Conference, MNC 2003
国/地域Japan
CityTokyo
Period03/10/2903/10/31

ASJC Scopus subject areas

  • ハードウェアとアーキテクチャ
  • 電子工学および電気工学

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