Stepwise pattern modification of neuronal network during cultivation using photo-thermal etching of agarose architecture

Yoshihiro Sugio; Hiroyuki Moriguchi; Ikurou Suzuki; Tomoyuki Kaneko; Kenji Yasuda; Yasuhiko Jimbo

doi:10.1109/IMNC.2003.1268776

Stepwise pattern modification of neuronal network during cultivation using photo-thermal etching of agarose architecture

Yoshihiro Sugio, Hiroyuki Moriguchi, Ikurou Suzuki, Tomoyuki Kaneko, Kenji Yasuda, Yasuhiko Jimbo

研究成果: Conference contribution

抄録

We have developed a new type of on-chip cell cultivation system using an agarose microchamber (AMC) array and a photo-thermal etching method, thus enabling topographical control of neuronal network pattern step-by-step during cell cultivation. By using photo-thermal etching (micro melting) method, the number of microtunnel connecting microchambers can be easily increased, even during cell cultivation, according to the progress of the neuronal network formation. To demonstrate the capability of this system for topographical control of network formation, we cultured hippocampal neurons in this AMC array. We found that the cells in microchambers made fiber connections through microtunnels. Furthermore the cells even made fiber connections through additional microtunnels fabricated during cultivation by photo-thermal etching. The results showed that the photo-thermal etching could be used during cultivation without damaging cells.

本文言語	English
ホスト出版物のタイトル	Digest of Papers - Microprocesses and Nanotechnology 2003 - 2003 International Microprocesses and Nanotechnology Conference, MNC 2003
出版社	Institute of Electrical and Electronics Engineers Inc.
ページ	322-323
ページ数	2
ISBN（電子版）	4891140402, 9784891140403
DOI	https://doi.org/10.1109/IMNC.2003.1268776
出版ステータス	Published - 2003
外部発表	はい
イベント	International Microprocesses and Nanotechnology Conference, MNC 2003 - Tokyo, Japan 継続期間: 2003 10月 29 → 2003 10月 31

Other

Other	International Microprocesses and Nanotechnology Conference, MNC 2003
国/地域	Japan
City	Tokyo
Period	03/10/29 → 03/10/31

ASJC Scopus subject areas

ハードウェアとアーキテクチャ
電子工学および電気工学

文献へのアクセス

10.1109/IMNC.2003.1268776

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引用スタイル

Sugio, Y., Moriguchi, H., Suzuki, I., Kaneko, T., Yasuda, K., & Jimbo, Y. (2003). Stepwise pattern modification of neuronal network during cultivation using photo-thermal etching of agarose architecture. In Digest of Papers - Microprocesses and Nanotechnology 2003 - 2003 International Microprocesses and Nanotechnology Conference, MNC 2003 (pp. 322-323). [1268776] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/IMNC.2003.1268776

Stepwise pattern modification of neuronal network during cultivation using photo-thermal etching of agarose architecture. / Sugio, Yoshihiro; Moriguchi, Hiroyuki; Suzuki, Ikurou; Kaneko, Tomoyuki; Yasuda, Kenji; Jimbo, Yasuhiko.

Digest of Papers - Microprocesses and Nanotechnology 2003 - 2003 International Microprocesses and Nanotechnology Conference, MNC 2003. Institute of Electrical and Electronics Engineers Inc., 2003. p. 322-323 1268776.

研究成果: Conference contribution

Sugio, Y, Moriguchi, H, Suzuki, I, Kaneko, T, Yasuda, K & Jimbo, Y 2003, Stepwise pattern modification of neuronal network during cultivation using photo-thermal etching of agarose architecture. in Digest of Papers - Microprocesses and Nanotechnology 2003 - 2003 International Microprocesses and Nanotechnology Conference, MNC 2003., 1268776, Institute of Electrical and Electronics Engineers Inc., pp. 322-323, International Microprocesses and Nanotechnology Conference, MNC 2003, Tokyo, Japan, 03/10/29. https://doi.org/10.1109/IMNC.2003.1268776

Sugio Y, Moriguchi H, Suzuki I, Kaneko T, Yasuda K, Jimbo Y. Stepwise pattern modification of neuronal network during cultivation using photo-thermal etching of agarose architecture. In Digest of Papers - Microprocesses and Nanotechnology 2003 - 2003 International Microprocesses and Nanotechnology Conference, MNC 2003. Institute of Electrical and Electronics Engineers Inc. 2003. p. 322-323. 1268776 https://doi.org/10.1109/IMNC.2003.1268776

Sugio, Yoshihiro ; Moriguchi, Hiroyuki ; Suzuki, Ikurou ; Kaneko, Tomoyuki ; Yasuda, Kenji ; Jimbo, Yasuhiko. / Stepwise pattern modification of neuronal network during cultivation using photo-thermal etching of agarose architecture. Digest of Papers - Microprocesses and Nanotechnology 2003 - 2003 International Microprocesses and Nanotechnology Conference, MNC 2003. Institute of Electrical and Electronics Engineers Inc., 2003. pp. 322-323

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abstract = "We have developed a new type of on-chip cell cultivation system using an agarose microchamber (AMC) array and a photo-thermal etching method, thus enabling topographical control of neuronal network pattern step-by-step during cell cultivation. By using photo-thermal etching (micro melting) method, the number of microtunnel connecting microchambers can be easily increased, even during cell cultivation, according to the progress of the neuronal network formation. To demonstrate the capability of this system for topographical control of network formation, we cultured hippocampal neurons in this AMC array. We found that the cells in microchambers made fiber connections through microtunnels. Furthermore the cells even made fiber connections through additional microtunnels fabricated during cultivation by photo-thermal etching. The results showed that the photo-thermal etching could be used during cultivation without damaging cells.",

author = "Yoshihiro Sugio and Hiroyuki Moriguchi and Ikurou Suzuki and Tomoyuki Kaneko and Kenji Yasuda and Yasuhiko Jimbo",

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AU - Yasuda, Kenji

AU - Jimbo, Yasuhiko

PY - 2003

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Stepwise pattern modification of neuronal network during cultivation using photo-thermal etching of agarose architecture

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