抄録
An analytical method was developed for determination of p-hydroxyphenylphenylhydantoin enantiomers in rat liver microsome by using reversed-phase high-performance liquid chromatography. A 50 mm C8 column was used as the analytical column. The mobile phase was made up of 8.8 mmol/L β-cyclodextrin, 0.25 mol/L urea and 0.05 mol/L ammonium acetate in water. The assay was linear from 2.05 to 410.0 μmol/L for each enantiomer. The limits of detection and of quantitation for the method were 0.90 and 2.05 μmol/L for each enantiomer, respectively. The analytical method afforded average recoveries of 93.59 ± 2.75% and 94.72 ± 1.78% for S- and R-p-hydroxyphenylphenylhydantoin, respectively. The method allowed study of the in vitro glucoronidation of p-hydroxyphenylphenylhydantoin in rat liver microsomal incubates. The stereoselectivity of p-hydroxyphenyl-phenylhydantoin phase II metabolism was observed.
本文言語 | English |
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ページ(範囲) | 141-144 |
ページ数 | 4 |
ジャーナル | Biomedical Chromatography |
巻 | 15 |
号 | 2 |
DOI | |
出版ステータス | Published - 2001 |
外部発表 | はい |
ASJC Scopus subject areas
- 分析化学
- 臨床生化学
- 生化学、遺伝学、分子生物学(全般)
- 生化学
- 薬理学