Stereoselective determination of p-hydroxyphenylphenylhydantoin enantiomers in rat liver microsomal incubates by reversed-phase high-performance liquid chromatography using β-cyclodextrin as chiral mobile phase additives

Akihiro Hattori, Takeshi Fukushima, Kimio Hamamura, Masaru Kato, Kazuhiro Imai

研究成果: Article査読

5 被引用数 (Scopus)

抄録

An analytical method was developed for determination of p-hydroxyphenylphenylhydantoin enantiomers in rat liver microsome by using reversed-phase high-performance liquid chromatography. A 50 mm C8 column was used as the analytical column. The mobile phase was made up of 8.8 mmol/L β-cyclodextrin, 0.25 mol/L urea and 0.05 mol/L ammonium acetate in water. The assay was linear from 2.05 to 410.0 μmol/L for each enantiomer. The limits of detection and of quantitation for the method were 0.90 and 2.05 μmol/L for each enantiomer, respectively. The analytical method afforded average recoveries of 93.59 ± 2.75% and 94.72 ± 1.78% for S- and R-p-hydroxyphenylphenylhydantoin, respectively. The method allowed study of the in vitro glucoronidation of p-hydroxyphenylphenylhydantoin in rat liver microsomal incubates. The stereoselectivity of p-hydroxyphenyl-phenylhydantoin phase II metabolism was observed.

本文言語English
ページ(範囲)141-144
ページ数4
ジャーナルBiomedical Chromatography
15
2
DOI
出版ステータスPublished - 2001
外部発表はい

ASJC Scopus subject areas

  • 分析化学
  • 臨床生化学
  • 生化学、遺伝学、分子生物学(全般)
  • 生化学
  • 薬理学

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