Stimulation of Dmc1-mediated DNA strand exchange by the human Rad54B protein

Naoyuki Sarai, Wataru Kagawa, Takashi Kinebuchi, Ako Kagawa, Kozo Tanaka, Kiyoshi Miyagawa, Shukuko Ikawa, Takehiko Shibata, Hitoshi Kurumizaka, Shigeyuki Yokoyama

    研究成果: Article

    16 引用 (Scopus)

    抜粋

    The process of homologous recombination is indispensable for both meiotic and mitotic cell division, and is one of the major pathways for double-strand break (DSB) repair. The human Rad54B protein, which belongs to the SWI2/SNF2 protein family, plays a role in homologous recombination, and may function with the Dmc1 recombinase, a meiosis-specific Rad51 homolog. In the present study, we found that Rad54B enhanced the DNA strand-exchange activity of Dmc1 by stabilizing the Dmc1-single-stranded DNA (ssDNA) complex. Therefore, Rad54B may stimulate the Dmc1-mediated DNA strand exchange by stabilizing the nucleoprotein filament, which is formed on the ssDNA tails produced at DSB sites during homologous recombination.

    元の言語English
    ページ(範囲)4429-4437
    ページ数9
    ジャーナルNucleic Acids Research
    34
    発行部数16
    DOI
    出版物ステータスPublished - 2006 9

      フィンガープリント

    ASJC Scopus subject areas

    • Genetics

    これを引用

    Sarai, N., Kagawa, W., Kinebuchi, T., Kagawa, A., Tanaka, K., Miyagawa, K., Ikawa, S., Shibata, T., Kurumizaka, H., & Yokoyama, S. (2006). Stimulation of Dmc1-mediated DNA strand exchange by the human Rad54B protein. Nucleic Acids Research, 34(16), 4429-4437. https://doi.org/10.1093/nar/gkl562