Stimulation of F1-ATPase activity by sodium dodecyl sulfate

Mohammad Delawar Hossain, Shou Furuike, Yasuhiro Onoue, Kengo Adachi, Masasuke Yoshida, Kazuhiko Kinosita

    研究成果: Article

    2 引用 (Scopus)

    抄録

    F1-ATPase is a rotary molecular motor in which the γ subunit rotates inside the cylinder made of α3Β3 subunits. We have studied the effects of sodium dodecyl sulfate (SDS) on the rotational and ATP hydrolysis activities of F1-ATPase. Bulk hydrolysis activity at various SDS concentrations was examined at 2mM ATP. Maximal stimulation was obtained at 0.003% (w/v) SDS, the initial (least inhibited) activity being about 1.4 times and the steady-state activity 3-4 times the values in the absence of SDS. Rotation rates observed with a 40-nm gold bead or a 0.29-μm bead duplex as well as the torque were unaffected by the presence of 0.003% SDS. The fraction of beads that rotated, in contrast, tended to increase in the presence of SDS. SDS seems to bring inactive F1 molecules into an active form but it does not alter or enhance the function of already active F1 molecules significantly.

    元の言語English
    ページ(範囲)435-442
    ページ数8
    ジャーナルBiochimica et Biophysica Acta - Bioenergetics
    1797
    発行部数4
    DOI
    出版物ステータスPublished - 2010 4

    Fingerprint

    Proton-Translocating ATPases
    Sodium Dodecyl Sulfate
    Hydrolysis
    Adenosine Triphosphate
    Molecules
    Torque
    Engine cylinders
    Gold

    ASJC Scopus subject areas

    • Biochemistry
    • Biophysics
    • Cell Biology

    これを引用

    Hossain, M. D., Furuike, S., Onoue, Y., Adachi, K., Yoshida, M., & Kinosita, K. (2010). Stimulation of F1-ATPase activity by sodium dodecyl sulfate. Biochimica et Biophysica Acta - Bioenergetics, 1797(4), 435-442. https://doi.org/10.1016/j.bbabio.2009.12.018

    Stimulation of F1-ATPase activity by sodium dodecyl sulfate. / Hossain, Mohammad Delawar; Furuike, Shou; Onoue, Yasuhiro; Adachi, Kengo; Yoshida, Masasuke; Kinosita, Kazuhiko.

    :: Biochimica et Biophysica Acta - Bioenergetics, 巻 1797, 番号 4, 04.2010, p. 435-442.

    研究成果: Article

    Hossain, MD, Furuike, S, Onoue, Y, Adachi, K, Yoshida, M & Kinosita, K 2010, 'Stimulation of F1-ATPase activity by sodium dodecyl sulfate', Biochimica et Biophysica Acta - Bioenergetics, 巻. 1797, 番号 4, pp. 435-442. https://doi.org/10.1016/j.bbabio.2009.12.018
    Hossain, Mohammad Delawar ; Furuike, Shou ; Onoue, Yasuhiro ; Adachi, Kengo ; Yoshida, Masasuke ; Kinosita, Kazuhiko. / Stimulation of F1-ATPase activity by sodium dodecyl sulfate. :: Biochimica et Biophysica Acta - Bioenergetics. 2010 ; 巻 1797, 番号 4. pp. 435-442.
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    AU - Adachi, Kengo

    AU - Yoshida, Masasuke

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