抄録
A sequential enzymatic incubation in collagenase and trypsin was carried out to yield a suspension of viable single cells from the seminiferous epithelium of adult human testis. The cell suspension predominantly consisted of pachytene primary spermatocytes (15%), round spermatids (32%), and condensing spermatids and residual bodies (21%). Human pachytene spermatocytes were isolated by unit gravity sedimentation using the methods originally developed for murine tissue. The spermatocyte-enriched fraction was 79% pure. When the effect of energy sources on protein synthesis by spermatocytes was examined, the highest rate of protein synthesis with pyruvate was found among four kinds of substrates added at a concentration of 10 mM. As shown with murine spermatocytes, the rate of protein synthesis by the human spermatocytes is probably regulated by pyruvate.
本文言語 | English |
---|---|
ページ(範囲) | 461-465 |
ページ数 | 5 |
ジャーナル | Andrologia |
巻 | 17 |
号 | 5 |
出版ステータス | Published - 1985 |
外部発表 | はい |
ASJC Scopus subject areas
- 内分泌学
- 腎臓病学