Effect of fructose 2, 6‐bisphosphate on 6‐phosphofructokinase (ATP: D‐fructose‐6‐phosphate 1‐phosphotransferase, EC 220.127.116.11) in spermatid extract from rat testes was studied. Fructose 2, 6‐bisphosphate stimulated the enzyme greatly by increasing its affinity for fructose 6‐phosphate and relieving the inhibition by ATP. Fructose 2, 6‐bisphosphate (0.8 μM) was required for 50% activation of 6‐phosphofructokinase (PFK). In addition, fructose 2, 6‐bisphosphate, AMP and fructose 6‐phosphate acted cooperatively to stimulate the activity of PFK. This stimulation may play an important role in the regulation of glycolysis in spermatids of rat testes.
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