Stochastic simulation of biological reactions, and its applications for studying actin polymerization

Kazuhisa Ichikawa, Takashi Suzuki, Noboru Murata

    研究成果: Article

    3 引用 (Scopus)

    抄録

    Molecular events in biological cells occur in local subregions, where the molecules tend to be small in number. The cytoskeleton, which is important for both the structural changes of cells and their functions, is also a countable entity because of its long fibrous shape. To simulate the local environment using a computer, stochastic simulations should be run. We herein report a new method of stochastic simulation based on random walk and reaction by the collision of all molecules. The microscopic reaction rate Pr is calculated from the macroscopic rate constant k. The formula involves only local parameters embedded for each molecule. The results of the stochastic simulations of simple second-order, polymerization, Michaelis-Menten-type and other reactions agreed quite well with those of deterministic simulations when the number of molecules was sufficiently large. An analysis of the theory indicated a relationship between variance and the number of molecules in the system, and results of multiple stochastic simulation runs confirmed this relationship. We simulated Ca2+ dynamics in a cell by inward flow from a point on the cell surface and the polymerization of G-actin forming F-actin. Our results showed that this theory and method can be used to simulate spatially inhomogeneous events.

    元の言語English
    記事番号046010
    ジャーナルPhysical Biology
    7
    発行部数4
    DOI
    出版物ステータスPublished - 2010 12

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    Polymerization
    Actins
    Cytoskeleton
    Computer Simulation

    ASJC Scopus subject areas

    • Biophysics
    • Molecular Biology
    • Cell Biology
    • Structural Biology

    これを引用

    Stochastic simulation of biological reactions, and its applications for studying actin polymerization. / Ichikawa, Kazuhisa; Suzuki, Takashi; Murata, Noboru.

    :: Physical Biology, 巻 7, 番号 4, 046010, 12.2010.

    研究成果: Article

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