Structural Basis for Actin Assembly, Activation of ATP Hydrolysis, and Delayed Phosphate Release

Kenji Murakami, Takuo Yasunaga, Taro Q.P. Noguchi, Yuki Gomibuchi, Kien X. Ngo, Taro Q.P. Uyeda, Takeyuki Wakabayashi*

*この研究の対応する著者

研究成果: Article査読

111 被引用数 (Scopus)

抄録

Assembled actin filaments support cellular signaling, intracellular trafficking, and cytokinesis. ATP hydrolysis triggered by actin assembly provides the structural cues for filament turnover in vivo. Here, we present the cryo-electron microscopic (cryo-EM) structure of filamentous actin (F-actin) in the presence of phosphate, with the visualization of some α-helical backbones and large side chains. A complete atomic model based on the EM map identified intermolecular interactions mediated by bound magnesium and phosphate ions. Comparison of the F-actin model with G-actin monomer crystal structures reveals a critical role for bending of the conserved proline-rich loop in triggering phosphate release following ATP hydrolysis. Crystal structures of G-actin show that mutations in this loop trap the catalytic site in two intermediate states of the ATPase cycle. The combined structural information allows us to propose a detailed molecular mechanism for the biochemical events, including actin polymerization and ATPase activation, critical for actin filament dynamics.

本文言語English
ページ(範囲)275-287
ページ数13
ジャーナルCell
143
2
DOI
出版ステータスPublished - 2010 10月 15
外部発表はい

ASJC Scopus subject areas

  • 生化学、遺伝学、分子生物学(全般)

フィンガープリント

「Structural Basis for Actin Assembly, Activation of ATP Hydrolysis, and Delayed Phosphate Release」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル