Structural basis of instability of the nucleosome containing a testis-specific histone variant, human H3T

Hiroaki Tachiwana, Wataru Kagawa, Akihisa Osakabe, Koichiro Kawaguchi, Tatsuya Shiga, Yoko Hayashi-Takanaka, Hiroshi Kimura, Hitoshi Kurumizaka*

*この研究の対応する著者

    研究成果: Article査読

    153 被引用数 (Scopus)

    抄録

    A histone H3 variant, H3T, is highly expressed in the testis, suggesting that it may play an important role in the chromatin reorganization required for meiosis and/or spermatogenesis. In the present study, we found that the nucleosome containing human H3T is significantly unstable both in vitro and in vivo, as compared to the conventional nucleosome containing H3.1. The crystal structure of the H3T nucleosome revealed structural differences in the H3T regions on both ends of the central α2 helix, as compared to those of H3.1. The H3T-specific residues (Met71 and Val111) are the source of the structural differences observed between H3T and H3.1. A mutational analysis revealed that these residues are responsible for the reduced stability of the H3T-containing nucleosome. These physical and structural properties of the H3T-containing nucleosome may provide the basis of chromatin reorganization during spermatogenesis.

    本文言語English
    ページ(範囲)10454-10459
    ページ数6
    ジャーナルProceedings of the National Academy of Sciences of the United States of America
    107
    23
    DOI
    出版ステータスPublished - 2010 6月 8

    ASJC Scopus subject areas

    • 一般

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