Structural changes in gonadal basement membranes during sex differentiation in the frog Rana rugosa

Kazuhiro Saotome, Tomoko Isomura, Tatsunori Seki, Yoriko Nakamura, Masahisa Nakamura*

*この研究の対応する著者

研究成果査読

13 被引用数 (Scopus)

抄録

Here we report that structural changes in gonadal basement membranes during sex differentiation in the frog Rana rugosa are revealed using an antibody to its laminin component. Immunohistochemical staining indicated that the first sexual dimorphism appeared in testicular cords and ovarian cavities in differentiating gonads of tadpoles at St. 25-3W, three weeks after they reached St. 25. During development, as the testis enlarged, testicular cord partitions appeared to form by invagination of the testicular epithelium. Ovarian cavities also increased in volume. Lamininpositive basement membranes initially surrounded a partial surface of oocytes close to the ovarian cavity, fully covering growing oocytes by St. X. Laminin-reactive signals were present in somatic cells outside seminiferous tubules in the testis and outside oocytes in one-year-old frogs. BrdUlabeling showed that the number of dividing germ cells increased continuously in male gonads but increased in females only up to St. V, declining at St. X and thereafter. The number of dividing germ cells declined when the basement membranes had fully covered the oocytes. Together, these findings suggest that the first sexual dimorphism in the gonad of R. rugosa first appears as a structural change in the basement membranes. Finally, we speculate that the basement membrane on the surface of oocytes may affect their proliferation in this species.

本文言語English
ページ(範囲)369-380
ページ数12
ジャーナルJournal of Experimental Zoology Part A: Ecological Genetics and Physiology
313 A
6
DOI
出版ステータスPublished - 2010 7 1

ASJC Scopus subject areas

  • 動物科学および動物学
  • 生態、進化、行動および分類学
  • 遺伝学
  • 生理学
  • 分子生物学

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