Structural compensation for the deficit of rRNA with proteins in the mammalian mitochondrial ribosome. Systematic analysis of protein components of the large ribosomal subunit from mammalian mitochondria

Tsutomu Suzuki, Maki Terasaki, Chie Takemoto-Hori, Takao Hanada, Takuya Ueda, Akira Wada, Kimitsuna Watanabe

研究成果: Article

91 引用 (Scopus)

抄録

The mammalian mitochondrial ribosome (mitoribosome) is a highly protein-rich particle in which almost half of the rRNA contained in the bacterial ribosome is replaced with proteins. It is known that mitochondrial translation factors can function on both mitochondrial and Escherichia coli ribosomes, indicating that protein components in the mitoribosome compensate the reduced rRNA chain to make a bacteria-type ribosome. To elucidate the molecular basis of this compensation, we analyzed bovine mitoribosomal large subunit proteins; 31 proteins were identified including 15 newly identified proteins with their cDNA sequences from human and mouse. The results showed that the proteins with binding sites on rRNA shortened or lost in the mitoribosome were enlarged when compared with the E. coli counterparts; this suggests the structural compensation of the rRNA deficit by the enlarged proteins in the mitoribosome.

元の言語English
ページ(範囲)21724-21736
ページ数13
ジャーナルJournal of Biological Chemistry
276
発行部数24
DOI
出版物ステータスPublished - 2001 6 15
外部発表Yes

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Large Ribosome Subunits
Mitochondria
Ribosomes
Proteins
Escherichia coli
Protein Subunits
Protein Binding
Mitochondrial Ribosomes
Compensation and Redress
Complementary DNA
Binding Sites
Bacteria

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

これを引用

Structural compensation for the deficit of rRNA with proteins in the mammalian mitochondrial ribosome. Systematic analysis of protein components of the large ribosomal subunit from mammalian mitochondria. / Suzuki, Tsutomu; Terasaki, Maki; Takemoto-Hori, Chie; Hanada, Takao; Ueda, Takuya; Wada, Akira; Watanabe, Kimitsuna.

:: Journal of Biological Chemistry, 巻 276, 番号 24, 15.06.2001, p. 21724-21736.

研究成果: Article

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AU - Terasaki, Maki

AU - Takemoto-Hori, Chie

AU - Hanada, Takao

AU - Ueda, Takuya

AU - Wada, Akira

AU - Watanabe, Kimitsuna

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