Structural organization and expression of the mouse gene encoding α-galactosidase A

Toshio Ohshima, Gary J. Murray, James W. Nagle, Jane M. Quirk, Matthias H. Kraus, Norman W. Barton, Roscoe O. Brady, Ashok B. Kulkarni

研究成果: Article

25 引用 (Scopus)

抄録

α-Galactosidase A (α-d-galactoside galactohydrolase, EC 3.2.1.22;αGalA) is a lysosomal enzyme that hydrolyses the α-d-galactosyl residues from glycosphingolipids. Fabry disease, an inherited X-linked recessive human metabolic disorder, results from a mutation in the αGalA gene at Xq22. As a prerequisite for generating a mouse model of Fabry disease by gene targeting, we have isolated and characterized the mouse αGalA gene and cDNA. A cloned mouse αGalA cDNA encoded a putative precursor protein of 419 amino acids (aa), including a 31-aa signal peptide (SP). The deduced aa sequence showed high homology (79%) with the human αtGalA protein. Nucleotide sequence analysis of genomic clones revealed that the overall structure and organization of the gene was very similar to that of human αGalA. All exon-intron splice junctions conformed to the GT/AG consensus sequence. Comparison of genomic and cDNA sequences revealed the ocurrence of two putative polyadenylation signals whose alternative use results in the two mouse αGalA transcripts of 1.4 and 3.6 kb. The 5′-flanking region of mouse αGalA had no typical TATA box. Several putative promoter-associated elements including Spl, AP1 and a potential cAMP-responsive element (CRE) were identified. Northern blot analysis revealed the widespread tissue distribution of mouse αGalA transcripts. Lower expression levels, however, were observed in some tissues, implying tissue-specific differences in αGalA promoter function.

元の言語English
ページ(範囲)277-280
ページ数4
ジャーナルGene
166
発行部数2
DOI
出版物ステータスPublished - 1995 12 12
外部発表Yes

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Galactosidases
Gene Expression
Fabry Disease
Complementary DNA
Genes
Amino Acids
Galactosides
Glycosphingolipids
TATA Box
Polyadenylation
Protein Precursors
Gene Targeting
5' Flanking Region
Consensus Sequence
Tissue Distribution
Protein Sorting Signals
Northern Blotting
Introns
Sequence Analysis
Amino Acid Sequence

ASJC Scopus subject areas

  • Genetics

これを引用

Ohshima, T., Murray, G. J., Nagle, J. W., Quirk, J. M., Kraus, M. H., Barton, N. W., ... Kulkarni, A. B. (1995). Structural organization and expression of the mouse gene encoding α-galactosidase A. Gene, 166(2), 277-280. https://doi.org/10.1016/0378-1119(95)00592-7

Structural organization and expression of the mouse gene encoding α-galactosidase A. / Ohshima, Toshio; Murray, Gary J.; Nagle, James W.; Quirk, Jane M.; Kraus, Matthias H.; Barton, Norman W.; Brady, Roscoe O.; Kulkarni, Ashok B.

:: Gene, 巻 166, 番号 2, 12.12.1995, p. 277-280.

研究成果: Article

Ohshima, T, Murray, GJ, Nagle, JW, Quirk, JM, Kraus, MH, Barton, NW, Brady, RO & Kulkarni, AB 1995, 'Structural organization and expression of the mouse gene encoding α-galactosidase A', Gene, 巻. 166, 番号 2, pp. 277-280. https://doi.org/10.1016/0378-1119(95)00592-7
Ohshima T, Murray GJ, Nagle JW, Quirk JM, Kraus MH, Barton NW その他. Structural organization and expression of the mouse gene encoding α-galactosidase A. Gene. 1995 12 12;166(2):277-280. https://doi.org/10.1016/0378-1119(95)00592-7
Ohshima, Toshio ; Murray, Gary J. ; Nagle, James W. ; Quirk, Jane M. ; Kraus, Matthias H. ; Barton, Norman W. ; Brady, Roscoe O. ; Kulkarni, Ashok B. / Structural organization and expression of the mouse gene encoding α-galactosidase A. :: Gene. 1995 ; 巻 166, 番号 2. pp. 277-280.
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abstract = "α-Galactosidase A (α-d-galactoside galactohydrolase, EC 3.2.1.22;αGalA) is a lysosomal enzyme that hydrolyses the α-d-galactosyl residues from glycosphingolipids. Fabry disease, an inherited X-linked recessive human metabolic disorder, results from a mutation in the αGalA gene at Xq22. As a prerequisite for generating a mouse model of Fabry disease by gene targeting, we have isolated and characterized the mouse αGalA gene and cDNA. A cloned mouse αGalA cDNA encoded a putative precursor protein of 419 amino acids (aa), including a 31-aa signal peptide (SP). The deduced aa sequence showed high homology (79{\%}) with the human αtGalA protein. Nucleotide sequence analysis of genomic clones revealed that the overall structure and organization of the gene was very similar to that of human αGalA. All exon-intron splice junctions conformed to the GT/AG consensus sequence. Comparison of genomic and cDNA sequences revealed the ocurrence of two putative polyadenylation signals whose alternative use results in the two mouse αGalA transcripts of 1.4 and 3.6 kb. The 5′-flanking region of mouse αGalA had no typical TATA box. Several putative promoter-associated elements including Spl, AP1 and a potential cAMP-responsive element (CRE) were identified. Northern blot analysis revealed the widespread tissue distribution of mouse αGalA transcripts. Lower expression levels, however, were observed in some tissues, implying tissue-specific differences in αGalA promoter function.",
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AU - Ohshima, Toshio

AU - Murray, Gary J.

AU - Nagle, James W.

AU - Quirk, Jane M.

AU - Kraus, Matthias H.

AU - Barton, Norman W.

AU - Brady, Roscoe O.

AU - Kulkarni, Ashok B.

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N2 - α-Galactosidase A (α-d-galactoside galactohydrolase, EC 3.2.1.22;αGalA) is a lysosomal enzyme that hydrolyses the α-d-galactosyl residues from glycosphingolipids. Fabry disease, an inherited X-linked recessive human metabolic disorder, results from a mutation in the αGalA gene at Xq22. As a prerequisite for generating a mouse model of Fabry disease by gene targeting, we have isolated and characterized the mouse αGalA gene and cDNA. A cloned mouse αGalA cDNA encoded a putative precursor protein of 419 amino acids (aa), including a 31-aa signal peptide (SP). The deduced aa sequence showed high homology (79%) with the human αtGalA protein. Nucleotide sequence analysis of genomic clones revealed that the overall structure and organization of the gene was very similar to that of human αGalA. All exon-intron splice junctions conformed to the GT/AG consensus sequence. Comparison of genomic and cDNA sequences revealed the ocurrence of two putative polyadenylation signals whose alternative use results in the two mouse αGalA transcripts of 1.4 and 3.6 kb. The 5′-flanking region of mouse αGalA had no typical TATA box. Several putative promoter-associated elements including Spl, AP1 and a potential cAMP-responsive element (CRE) were identified. Northern blot analysis revealed the widespread tissue distribution of mouse αGalA transcripts. Lower expression levels, however, were observed in some tissues, implying tissue-specific differences in αGalA promoter function.

AB - α-Galactosidase A (α-d-galactoside galactohydrolase, EC 3.2.1.22;αGalA) is a lysosomal enzyme that hydrolyses the α-d-galactosyl residues from glycosphingolipids. Fabry disease, an inherited X-linked recessive human metabolic disorder, results from a mutation in the αGalA gene at Xq22. As a prerequisite for generating a mouse model of Fabry disease by gene targeting, we have isolated and characterized the mouse αGalA gene and cDNA. A cloned mouse αGalA cDNA encoded a putative precursor protein of 419 amino acids (aa), including a 31-aa signal peptide (SP). The deduced aa sequence showed high homology (79%) with the human αtGalA protein. Nucleotide sequence analysis of genomic clones revealed that the overall structure and organization of the gene was very similar to that of human αGalA. All exon-intron splice junctions conformed to the GT/AG consensus sequence. Comparison of genomic and cDNA sequences revealed the ocurrence of two putative polyadenylation signals whose alternative use results in the two mouse αGalA transcripts of 1.4 and 3.6 kb. The 5′-flanking region of mouse αGalA had no typical TATA box. Several putative promoter-associated elements including Spl, AP1 and a potential cAMP-responsive element (CRE) were identified. Northern blot analysis revealed the widespread tissue distribution of mouse αGalA transcripts. Lower expression levels, however, were observed in some tissues, implying tissue-specific differences in αGalA promoter function.

KW - exon

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KW - intron

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