Structural properties and RNA-binding activities of two RNA recognition motifs of a mouse neural RNA-binding protein, mouse-Musashi-1

Yasuyuki Kurihara, Takashi Nagata, Takao Imai, Ado Hiwatashi, Masataka Horiuchi, Shinichi Sakakibara, Masato Katahira, Hideyuki Okano, Seiichi Uesugi

研究成果: Article

16 引用 (Scopus)

抄録

mouse-Musashi-1 (m-Msi-1) is an RNA-binding protein, abundantly expressed in the developing mammalian central nervous system (CNS). m-Msi-1 contains two RNA recognition motifs (RRMs). In this study, we found that the N-terminal RRM of m-Msi-1 (MMA) binds strongly to poly(G) and weakly to poly(U) in a way similar to that of the full-length m-Msi-1 protein characterized previously. The C-terminal RRM of m-Msi-1 (MMB), however, does not bind to RNA. In addition, the circular dichroism (CD) spectra of the two RRMs showed that the α-helical content of MMA is significantly higher than that of MMB, indicating that some differences in the secondary structure may be responsible for the distinct RNA binding properties of MMA and MMB.

元の言語English
ページ(範囲)21-27
ページ数7
ジャーナルGene
186
発行部数1
DOI
出版物ステータスPublished - 1997 2 20
外部発表Yes

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RNA-Binding Proteins
RNA
Poly G
Poly U
Circular Dichroism
Central Nervous System
RNA Recognition Motif

ASJC Scopus subject areas

  • Genetics

これを引用

Structural properties and RNA-binding activities of two RNA recognition motifs of a mouse neural RNA-binding protein, mouse-Musashi-1. / Kurihara, Yasuyuki; Nagata, Takashi; Imai, Takao; Hiwatashi, Ado; Horiuchi, Masataka; Sakakibara, Shinichi; Katahira, Masato; Okano, Hideyuki; Uesugi, Seiichi.

:: Gene, 巻 186, 番号 1, 20.02.1997, p. 21-27.

研究成果: Article

Kurihara, Y, Nagata, T, Imai, T, Hiwatashi, A, Horiuchi, M, Sakakibara, S, Katahira, M, Okano, H & Uesugi, S 1997, 'Structural properties and RNA-binding activities of two RNA recognition motifs of a mouse neural RNA-binding protein, mouse-Musashi-1', Gene, 巻. 186, 番号 1, pp. 21-27. https://doi.org/10.1016/S0378-1119(96)00673-7
Kurihara, Yasuyuki ; Nagata, Takashi ; Imai, Takao ; Hiwatashi, Ado ; Horiuchi, Masataka ; Sakakibara, Shinichi ; Katahira, Masato ; Okano, Hideyuki ; Uesugi, Seiichi. / Structural properties and RNA-binding activities of two RNA recognition motifs of a mouse neural RNA-binding protein, mouse-Musashi-1. :: Gene. 1997 ; 巻 186, 番号 1. pp. 21-27.
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abstract = "mouse-Musashi-1 (m-Msi-1) is an RNA-binding protein, abundantly expressed in the developing mammalian central nervous system (CNS). m-Msi-1 contains two RNA recognition motifs (RRMs). In this study, we found that the N-terminal RRM of m-Msi-1 (MMA) binds strongly to poly(G) and weakly to poly(U) in a way similar to that of the full-length m-Msi-1 protein characterized previously. The C-terminal RRM of m-Msi-1 (MMB), however, does not bind to RNA. In addition, the circular dichroism (CD) spectra of the two RRMs showed that the α-helical content of MMA is significantly higher than that of MMB, indicating that some differences in the secondary structure may be responsible for the distinct RNA binding properties of MMA and MMB.",
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AU - Hiwatashi, Ado

AU - Horiuchi, Masataka

AU - Sakakibara, Shinichi

AU - Katahira, Masato

AU - Okano, Hideyuki

AU - Uesugi, Seiichi

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