Structural uniformity of pullulan produced by several strains of pullularia pullulans

Ryo Taguchi, Yo Kikuchi, Yoshiyuki Sakano, Tsuneo Kobayashi

研究成果: Article査読

65 被引用数 (Scopus)


Extracellular polysaccharides produced by 3 strains of Pullularia pullulans were fractionated by treating with cetyl trimethylammonium hydroxide into soluble and insoluble fractions, and the structure of the former fraction, i.e., pullulan, was studied. The yield and the ratio of 2 fractions varied widely according to the strains. But the structure of pullulan was found to be uniform irrespective of the strains used. All 3 samples of pullulan gave only glucose on complete acid hydrolysis, and 93-95% maltotriose and 5-7% maltotetraose after isoamylase (pullulanase) action. The ratio of a-1, 4- to a-1, 6-glucosidic linkages calculated from periodate oxidation data coincided very well with the value expected from the ratio of maltotriose to maltotetraose units. An evidence for the complete absence of branch structure in pullulan was presented from the results of hydrolysis by pullulan 4-glucanohydrolase.

ジャーナルAgricultural and Biological Chemistry
出版ステータスPublished - 1973

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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