抄録
Extracellular polysaccharides produced by 3 strains of Pullularia pullulans were fractionated by treating with cetyl trimethylammonium hydroxide into soluble and insoluble fractions, and the structure of the former fraction, i.e., pullulan, was studied. The yield and the ratio of 2 fractions varied widely according to the strains. But the structure of pullulan was found to be uniform irrespective of the strains used. All 3 samples of pullulan gave only glucose on complete acid hydrolysis, and 93~95% maltotriose and 5~7% maltotetraose after isoamylase (pullulanase) action. The ratio of α-1, 4- to α-1, 6-glucosidic linkages calculated from periodate oxidation data coincided very well with the value expected from the ratio of maltotriose to maltotetraose units. An evidence for the complete absence of branch structure in pullulan was presented from the results of hydrolysis by pullulan 4-glucanohydrolase.
| 元の言語 | English |
|---|---|
| ページ（範囲） | 1583-1588 |
| ページ数 | 6 |
| ジャーナル | Agricultural and Biological Chemistry |
| 巻 | 37 |
| 発行部数 | 7 |
| DOI | |
| 出版物ステータス | Published - 1973 |
| 外部発表 | Yes |
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ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Agricultural and Biological Sciences(all)
これを引用
Structural Uniformity of Pullulan Produced by Several Strains of Pullularia pullulans. / Taguchi, Ryo; Kikuchi, Yo; Sakano, Yoshiyuki; Kobayashi, Tsuneo.
：: Agricultural and Biological Chemistry, 巻 37, 番号 7, 1973, p. 1583-1588.研究成果: Article
}
TY - JOUR
T1 - Structural Uniformity of Pullulan Produced by Several Strains of Pullularia pullulans
AU - Taguchi, Ryo
AU - Kikuchi, Yo
AU - Sakano, Yoshiyuki
AU - Kobayashi, Tsuneo
PY - 1973
Y1 - 1973
N2 - Extracellular polysaccharides produced by 3 strains of Pullularia pullulans were fractionated by treating with cetyl trimethylammonium hydroxide into soluble and insoluble fractions, and the structure of the former fraction, i.e., pullulan, was studied. The yield and the ratio of 2 fractions varied widely according to the strains. But the structure of pullulan was found to be uniform irrespective of the strains used. All 3 samples of pullulan gave only glucose on complete acid hydrolysis, and 93~95% maltotriose and 5~7% maltotetraose after isoamylase (pullulanase) action. The ratio of α-1, 4- to α-1, 6-glucosidic linkages calculated from periodate oxidation data coincided very well with the value expected from the ratio of maltotriose to maltotetraose units. An evidence for the complete absence of branch structure in pullulan was presented from the results of hydrolysis by pullulan 4-glucanohydrolase.
AB - Extracellular polysaccharides produced by 3 strains of Pullularia pullulans were fractionated by treating with cetyl trimethylammonium hydroxide into soluble and insoluble fractions, and the structure of the former fraction, i.e., pullulan, was studied. The yield and the ratio of 2 fractions varied widely according to the strains. But the structure of pullulan was found to be uniform irrespective of the strains used. All 3 samples of pullulan gave only glucose on complete acid hydrolysis, and 93~95% maltotriose and 5~7% maltotetraose after isoamylase (pullulanase) action. The ratio of α-1, 4- to α-1, 6-glucosidic linkages calculated from periodate oxidation data coincided very well with the value expected from the ratio of maltotriose to maltotetraose units. An evidence for the complete absence of branch structure in pullulan was presented from the results of hydrolysis by pullulan 4-glucanohydrolase.
UR - http://www.scopus.com/inward/record.url?scp=85004560066&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85004560066&partnerID=8YFLogxK
U2 - 10.1271/bbb1961.37.1583
DO - 10.1271/bbb1961.37.1583
M3 - Article
AN - SCOPUS:85004560066
VL - 37
SP - 1583
EP - 1588
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
SN - 0916-8451
IS - 7
ER -