Structural Uniformity of Pullulan Produced by Several Strains of Pullularia pullulans

Ryo Taguchi, Yo Kikuchi, Yoshiyuki Sakano, Tsuneo Kobayashi

研究成果: Article

2 引用 (Scopus)

抄録

Extracellular polysaccharides produced by 3 strains of Pullularia pullulans were fractionated by treating with cetyl trimethylammonium hydroxide into soluble and insoluble fractions, and the structure of the former fraction, i.e., pullulan, was studied. The yield and the ratio of 2 fractions varied widely according to the strains. But the structure of pullulan was found to be uniform irrespective of the strains used. All 3 samples of pullulan gave only glucose on complete acid hydrolysis, and 93~95% maltotriose and 5~7% maltotetraose after isoamylase (pullulanase) action. The ratio of α-1, 4- to α-1, 6-glucosidic linkages calculated from periodate oxidation data coincided very well with the value expected from the ratio of maltotriose to maltotetraose units. An evidence for the complete absence of branch structure in pullulan was presented from the results of hydrolysis by pullulan 4-glucanohydrolase.

元の言語English
ページ(範囲)1583-1588
ページ数6
ジャーナルAgricultural and Biological Chemistry
37
発行部数7
DOI
出版物ステータスPublished - 1973
外部発表Yes

Fingerprint

pullulan
Aureobasidium pullulans
maltotriose
isoamylase
pullulanase
Hydrolysis
Isoamylase
hydroxides
acid hydrolysis
linkage (genetics)
polysaccharides
hydrolysis
Polysaccharides
oxidation
glucose
Glucose
Oxidation
Acids

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

これを引用

Structural Uniformity of Pullulan Produced by Several Strains of Pullularia pullulans. / Taguchi, Ryo; Kikuchi, Yo; Sakano, Yoshiyuki; Kobayashi, Tsuneo.

:: Agricultural and Biological Chemistry, 巻 37, 番号 7, 1973, p. 1583-1588.

研究成果: Article

Taguchi, Ryo ; Kikuchi, Yo ; Sakano, Yoshiyuki ; Kobayashi, Tsuneo. / Structural Uniformity of Pullulan Produced by Several Strains of Pullularia pullulans. :: Agricultural and Biological Chemistry. 1973 ; 巻 37, 番号 7. pp. 1583-1588.
@article{e756d9cf3cf343578cb32e1cd86518b9,
title = "Structural Uniformity of Pullulan Produced by Several Strains of Pullularia pullulans",
abstract = "Extracellular polysaccharides produced by 3 strains of Pullularia pullulans were fractionated by treating with cetyl trimethylammonium hydroxide into soluble and insoluble fractions, and the structure of the former fraction, i.e., pullulan, was studied. The yield and the ratio of 2 fractions varied widely according to the strains. But the structure of pullulan was found to be uniform irrespective of the strains used. All 3 samples of pullulan gave only glucose on complete acid hydrolysis, and 93~95{\%} maltotriose and 5~7{\%} maltotetraose after isoamylase (pullulanase) action. The ratio of α-1, 4- to α-1, 6-glucosidic linkages calculated from periodate oxidation data coincided very well with the value expected from the ratio of maltotriose to maltotetraose units. An evidence for the complete absence of branch structure in pullulan was presented from the results of hydrolysis by pullulan 4-glucanohydrolase.",
author = "Ryo Taguchi and Yo Kikuchi and Yoshiyuki Sakano and Tsuneo Kobayashi",
year = "1973",
doi = "10.1271/bbb1961.37.1583",
language = "English",
volume = "37",
pages = "1583--1588",
journal = "Bioscience, Biotechnology and Biochemistry",
issn = "0916-8451",
publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",
number = "7",

}

TY - JOUR

T1 - Structural Uniformity of Pullulan Produced by Several Strains of Pullularia pullulans

AU - Taguchi, Ryo

AU - Kikuchi, Yo

AU - Sakano, Yoshiyuki

AU - Kobayashi, Tsuneo

PY - 1973

Y1 - 1973

N2 - Extracellular polysaccharides produced by 3 strains of Pullularia pullulans were fractionated by treating with cetyl trimethylammonium hydroxide into soluble and insoluble fractions, and the structure of the former fraction, i.e., pullulan, was studied. The yield and the ratio of 2 fractions varied widely according to the strains. But the structure of pullulan was found to be uniform irrespective of the strains used. All 3 samples of pullulan gave only glucose on complete acid hydrolysis, and 93~95% maltotriose and 5~7% maltotetraose after isoamylase (pullulanase) action. The ratio of α-1, 4- to α-1, 6-glucosidic linkages calculated from periodate oxidation data coincided very well with the value expected from the ratio of maltotriose to maltotetraose units. An evidence for the complete absence of branch structure in pullulan was presented from the results of hydrolysis by pullulan 4-glucanohydrolase.

AB - Extracellular polysaccharides produced by 3 strains of Pullularia pullulans were fractionated by treating with cetyl trimethylammonium hydroxide into soluble and insoluble fractions, and the structure of the former fraction, i.e., pullulan, was studied. The yield and the ratio of 2 fractions varied widely according to the strains. But the structure of pullulan was found to be uniform irrespective of the strains used. All 3 samples of pullulan gave only glucose on complete acid hydrolysis, and 93~95% maltotriose and 5~7% maltotetraose after isoamylase (pullulanase) action. The ratio of α-1, 4- to α-1, 6-glucosidic linkages calculated from periodate oxidation data coincided very well with the value expected from the ratio of maltotriose to maltotetraose units. An evidence for the complete absence of branch structure in pullulan was presented from the results of hydrolysis by pullulan 4-glucanohydrolase.

UR - http://www.scopus.com/inward/record.url?scp=85004560066&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85004560066&partnerID=8YFLogxK

U2 - 10.1271/bbb1961.37.1583

DO - 10.1271/bbb1961.37.1583

M3 - Article

AN - SCOPUS:85004560066

VL - 37

SP - 1583

EP - 1588

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

SN - 0916-8451

IS - 7

ER -