Structure and Expression of Novel Protein-Tyrosine Kinases, EMB and EMT, in Hematopoietic Cells

Nobuo Yamada, Yuko Kawakami, Hitoshi Kimura, Hiromi Fukamachi, Gottfried Baier, Amnon Altman, Takashi Kato, Yoshimasa Inagaki, Toshiaki Kawakami*

*この研究の対応する著者

研究成果: Article査読

115 被引用数 (Scopus)

抄録

Two novel tyrosine kinase cDNAs were obtained from murine mast cells. These kinases, Emb and Emt, constitute a novel tyrosine kinase subfamily which may also include Tec, a kinase preferentially expressed in liver, and Dsrc28, a fruit fly kinase. Both lack hydrophobic stretches characteristic of the transmembrane domains found in growth factor receptor tyrosine kinases and carboxyl-terminal, negative regulatory tyrosine residue found in Src family kinases. In addition to the Src homology region 2 (SH2) and SH3 domains characteristic of the Src family kinases and other signaling molecules, Emb and Emt share a similar amino-terminal domain comprised mainly of two repeat segments. The emb 2.7-kb transcript was expressed in mast cells, myeloid cells and B lymphocytes while the emt 4.6-kb mRNA in mast cells, myeloid cells and T lymphocytes. The evidence for in vitro tyrosine kinase activity of Emb and Emt proteins is also provided.

本文言語English
ページ(範囲)231-240
ページ数10
ジャーナルBiochemical and Biophysical Research Communications
192
1
DOI
出版ステータスPublished - 1993 1 1
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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