Structure of human nucleosome containing the testis-specific histone variant TSH2B

Takashi Urahama, Naoki Horikoshi, Akihisa Osakabe, Hiroaki Tachiwana, Hitoshi Kurumizaka

研究成果: Article

13 引用 (Scopus)

抄録

The human histone H2B variant TSH2B is highly expressed in testis and may function in the chromatin transition during spermatogenesis. In the present study, the crystal structure of the human testis-specific nucleosome containing TSH2B was determined at 2.8 Å resolution. A local structural difference between TSH2B and canonical H2B in nucleosomes was detected around the TSH2B-specific amino-acid residue Ser85. The TSH2B Ser85 residue does not interact with H4 in the nucleosome, but in the canonical nucleosome the H2B Asn84 residue (corresponding to the TSH2B Ser85 residue) forms water-mediated hydrogen bonds with the H4 Arg78 residue. In contrast, the other TSH2B-specific amino-acid residues did not induce any significant local structural changes in the TSH2B nucleosome. These findings may provide important information for understanding how testis-specific histone variants form nucleosomes during spermatogenesis.

元の言語English
ページ(範囲)444-449
ページ数6
ジャーナルActa Crystallographica Section F:Structural Biology Communications
70
発行部数4
DOI
出版物ステータスPublished - 2014

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testes
Nucleosomes
Histones
Testis
spermatogenesis
Spermatogenesis
amino acids
Amino Acids
chromatin
Chromatin
Hydrogen
Hydrogen bonds
Crystal structure
hydrogen bonds
crystal structure
Water
water

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Genetics
  • Structural Biology
  • Condensed Matter Physics
  • Medicine(all)

これを引用

Structure of human nucleosome containing the testis-specific histone variant TSH2B. / Urahama, Takashi; Horikoshi, Naoki; Osakabe, Akihisa; Tachiwana, Hiroaki; Kurumizaka, Hitoshi.

:: Acta Crystallographica Section F:Structural Biology Communications, 巻 70, 番号 4, 2014, p. 444-449.

研究成果: Article

Urahama, Takashi ; Horikoshi, Naoki ; Osakabe, Akihisa ; Tachiwana, Hiroaki ; Kurumizaka, Hitoshi. / Structure of human nucleosome containing the testis-specific histone variant TSH2B. :: Acta Crystallographica Section F:Structural Biology Communications. 2014 ; 巻 70, 番号 4. pp. 444-449.
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